TY - JOUR
T1 - Octopaminergic neurons have multiple targets in Drosophila larval mushroom body calyx and can modulate behavioral odor discrimination
AU - Wong, J. Y. Hilary
AU - Wan, Bo Angela
AU - Bland, Tom
AU - Montagnese, Marcella
AU - McLachlan, Alex D.
AU - O'Kane, Cahir J.
AU - Zhang, Shuo Wei
AU - Masuda-Nakagawa, Liria M.
N1 - Funding Information:
We thank B. Gerber for odor containers; A. Cardona for access and training on CATMAID; T. Awasaki, S. Certel, K. Ito, M Landgraf, T. Lee, C.H.-Lee, B Pfeiffer, M. Ramaswami, K. Scott, J. Truman, and the Bloomington Drosophila Stock Center (BDSC) for numerous fly stocks; the Cambridge Genetics Department Fly Facility for stocks and microinjections; and the Developmental Studies Hybridoma Bank (DSHB), University of Iowa, for antibodies. We thank M. Morgan for help in building optogenetic illumination apparatus. J.Y.H.W. was supported by a Medical Research Council Studentship, and M.M. was supported by a UK Genetics Society “Genes & Development” summer scholarship and an award from the Bedford Fund, King’s College Cambridge. T.B. was funded by a Biotechnology and Biological Sciences Research Council (BBSRC) Doctoral Training Program summer placement grant. This work was supported by BBSRC grants BB/I022651/1 and BB/ N007948/1, and an Isaac Newton Trust award to L.M.M.-N. and C.J.O.
Funding Information:
We thank B. Gerber for odor containers; A. Cardona for access and training on CATMAID; T. Awasaki, S. Certel, K. Ito, M Landgraf, T. Lee, C.H.-Lee, B Pfeiffer, M. Ramaswami, K. Scott, J. Truman, and the Bloomington Drosophila Stock Center (BDSC) for numerous fly stocks; the Cambridge Genetics Department Fly Facility for stocks and microinjections; and the Developmental Studies Hybridoma Bank (DSHB), University of Iowa, for antibodies. We thank M. Morgan for help in building optogenetic illumination apparatus. J.Y.H.W. was supported by a Medical Research Council Studentship, and M.M. was supported by a UK Genetics Society "Genes & Development" summer scholarship and an award from the Bedford Fund, King's College Cambridge. T.B. was funded by a Biotechnology and Biological Sciences Research Council (BBSRC) Doctoral Training Program summer placement grant. This work was supported by BBSRC grants BB/I022651/1 and BB/ N007948/1, and an Isaac Newton Trust award to L.M.M.-N. and C.J.O.
Publisher Copyright:
© 2021 Wong et al.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2
Y1 - 2021/2
N2 - Discrimination of sensory signals is essential for an organism to form and retrieve memories of relevance in a given behavioral context. Sensory representations are modified dynamically by changes in behavioral state, facilitating context-dependent selection of behavior, through signals carried by noradrenergic input in mammals, or octopamine (OA) in insects. To understand the circuit mechanisms of this signaling, we characterized the function of two OA neurons, sVUM1 neurons, that originate in the subesophageal zone (SEZ) and target the input region of the memory center, the mushroom body (MB) calyx, in larval Drosophila. We found that sVUM1 neurons target multiple neurons, including olfactory projection neurons (PNs), the inhibitory neuron APL, and a pair of extrinsic output neurons, but relatively few mushroom body intrinsic neurons, Kenyon cells. PN terminals carried the OA receptor Oamb, a Drosophila α1-adrenergic receptor ortholog. Using an odor discrimination learning paradigm, we showed that optogenetic activation of OA neurons compromised discrimination of similar odors but not learning ability. Our results suggest that sVUM1 neurons modify odor representations via multiple extrinsic inputs at the sensory input area to the MB olfactory learning circuit.
AB - Discrimination of sensory signals is essential for an organism to form and retrieve memories of relevance in a given behavioral context. Sensory representations are modified dynamically by changes in behavioral state, facilitating context-dependent selection of behavior, through signals carried by noradrenergic input in mammals, or octopamine (OA) in insects. To understand the circuit mechanisms of this signaling, we characterized the function of two OA neurons, sVUM1 neurons, that originate in the subesophageal zone (SEZ) and target the input region of the memory center, the mushroom body (MB) calyx, in larval Drosophila. We found that sVUM1 neurons target multiple neurons, including olfactory projection neurons (PNs), the inhibitory neuron APL, and a pair of extrinsic output neurons, but relatively few mushroom body intrinsic neurons, Kenyon cells. PN terminals carried the OA receptor Oamb, a Drosophila α1-adrenergic receptor ortholog. Using an odor discrimination learning paradigm, we showed that optogenetic activation of OA neurons compromised discrimination of similar odors but not learning ability. Our results suggest that sVUM1 neurons modify odor representations via multiple extrinsic inputs at the sensory input area to the MB olfactory learning circuit.
UR - http://www.scopus.com/inward/record.url?scp=85100125215&partnerID=8YFLogxK
U2 - 10.1101/lm.052159.120
DO - 10.1101/lm.052159.120
M3 - Article
SN - 1072-0502
VL - 28
SP - 53
EP - 71
JO - Learning and Memory
JF - Learning and Memory
IS - 2
ER -