Online Raman spectroscopy for Structural Biology on beamline ID29 of the ESRF

David von Stetten, Thierry Giraud, Soi Bui, Roberto A. Steiner, François Fihman, Daniele de Sanctis, Antoine Royant

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
316 Downloads (Pure)

Abstract

Raman spectroscopy can probe the structure and conformations of specific chemical groups within proteins and may thus be used as a technique complementary to X-ray crystallography. This combined approach can be decisive in resolving ambiguities in the interpretation of enzymatic or X-ray induced processes. Here, we present an online Raman setup developed at the European Synchrotron that allows for interleaved Raman spectra acquisition and X-ray diffraction measurements with fast probe exchange and simple alignment while maintaining a high sensitivity over the entire spectral range. This device has been recently employed in the study of a covalent intermediate in the O2-dependent breakdown of uric acid by the cofactor-free enzyme urate oxidase and to monitor its decay induced by X-ray exposure.
Original languageEnglish
Pages (from-to)124-127
JournalJournal of Structural Biology
Volume200
Issue number2
Early online date16 Oct 2017
DOIs
Publication statusE-pub ahead of print - 16 Oct 2017

Keywords

  • Raman spectroscopy
  • Macromolecular crystallography
  • Diffraction-complementary technique
  • Kinetic crystallography
  • Radiation damage

Fingerprint

Dive into the research topics of 'Online Raman spectroscopy for Structural Biology on beamline ID29 of the ESRF'. Together they form a unique fingerprint.

Cite this