Abstract
Introduction: The National Institute for Health and Care Excellence recommend the Triage PlGF (Alere) test and the Elecsys (Roche) sFlt1/PlGF ratio immunoassay to help ruleout preeclampsia in women presenting with suspected preeclampsia. The DELFIA Xpress PlGF 123 (Perkin Elmer) test is not currently recommended.
Objectives: To compare the performance of three commercially available PlGF kits in women with suspected or confirmed pre-eclampsia.
Participants and methods: Samples from 200 women with suspected or confirmed pre-eclampsia were analysed using the DELFIA Xpress PlGF 1–2-3 test (PerkinElmer), Elecsys immunoassay sFlt-1/PlGF ratio (Roche) and Triage PlGF test (Alere). Plasma and serum were assayed for PlGF and/or sFlt for the DELFIA and Elecsys assays and plasma alone for Triage PlGF. Pitman’s test of difference was used to assess variance between assays.
Results: Results are presented in Table 1. Bland–Altman comparison showed no significant difference in variance of serum concentrations between DELFIA and Elecsys assays (Pitman’s Test of difference in variance: r = 0.015, p = 0.832). The Triage assay (Alere) was found to have higher variance than both Elecys (Roche); r = −0.685, p < 0.001 and DELFIA (Perkin Elmer); r = −0.605, p < 0.001. There was relatively good agreement between PlGF measurements by EDTA and Serum using Roche: average relative difference of 12.6%, Bland–Altman reference range −9.2% to 39.5; for DELFIA, the relative difference was similar (11.1%), but the reference range was wider: −48.5% to 139.1%.
Conclusion: There was variance in PlGF concentrations across platforms. This may be explained by the measurement of differing isoforms of PlGF.
Objectives: To compare the performance of three commercially available PlGF kits in women with suspected or confirmed pre-eclampsia.
Participants and methods: Samples from 200 women with suspected or confirmed pre-eclampsia were analysed using the DELFIA Xpress PlGF 1–2-3 test (PerkinElmer), Elecsys immunoassay sFlt-1/PlGF ratio (Roche) and Triage PlGF test (Alere). Plasma and serum were assayed for PlGF and/or sFlt for the DELFIA and Elecsys assays and plasma alone for Triage PlGF. Pitman’s test of difference was used to assess variance between assays.
Results: Results are presented in Table 1. Bland–Altman comparison showed no significant difference in variance of serum concentrations between DELFIA and Elecsys assays (Pitman’s Test of difference in variance: r = 0.015, p = 0.832). The Triage assay (Alere) was found to have higher variance than both Elecys (Roche); r = −0.685, p < 0.001 and DELFIA (Perkin Elmer); r = −0.605, p < 0.001. There was relatively good agreement between PlGF measurements by EDTA and Serum using Roche: average relative difference of 12.6%, Bland–Altman reference range −9.2% to 39.5; for DELFIA, the relative difference was similar (11.1%), but the reference range was wider: −48.5% to 139.1%.
Conclusion: There was variance in PlGF concentrations across platforms. This may be explained by the measurement of differing isoforms of PlGF.
| Original language | English |
|---|---|
| Pages (from-to) | 16 |
| Number of pages | 1 |
| Journal | Pregnancy Hypertension |
| Volume | 9 |
| DOIs | |
| Publication status | E-pub ahead of print - 12 Aug 2017 |