Optical properties of human brain and tumour tissue: An ex vivo study spanning the visible range to beyond the second near-infrared window

Jonathan Shapey, Yijing Xie, Elham Nabavi, Michael Ebner, Shakeel R Saeed, Neil Kitchen, Neil Dorward, Joan Grieve, Andrew W McEvoy, Anna Miserocchi, Patrick Grover, Robert Bradford, Yau-Mun Lim, Sebastien Ourselin, Sebastian Brandner, Zane Jaunmuktane, Tom Vercauteren

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)
263 Downloads (Pure)

Abstract

Neuro-oncology surgery would benefit from detailed intraoperative tissue characterization provided by noncontact, contrast-agent-free, noninvasive optical imaging methods. In-depth knowledge of target tissue optical properties across a wide-wavelength spectrum could inform the design of optical imaging and computational methods to enable robust tissue analysis during surgery. We adapted a dual-beam integrating sphere to analyse small tissue samples and investigated ex vivo optical properties of five types of human brain tumour (meningioma, pituitary adenoma, schwannoma, low- and high-grade glioma) and nine different types of healthy brain tissue across a wavelength spectrum of 400 to 1800 nm. Fresh and frozen tissue samples were analysed. All tissue types demonstrated similar absorption spectra, but the reduced scattering coefficients of tumours show visible differences in the obtained optical spectrum compared to those of surrounding normal tissue. These results underline the potential of optical imaging technologies for intraoperative tissue characterization.

Original languageEnglish
Article numbere202100072
JournalJournal of biophotonics
Volume15
Issue number4
Early online date12 Feb 2022
DOIs
Publication statusPublished - Apr 2022

Fingerprint

Dive into the research topics of 'Optical properties of human brain and tumour tissue: An ex vivo study spanning the visible range to beyond the second near-infrared window'. Together they form a unique fingerprint.

Cite this