Optical properties of human brain and tumour tissue: An ex vivo study spanning the visible range to beyond the second near-infrared window

TY - JOUR

T1 - Optical properties of human brain and tumour tissue

T2 - An ex vivo study spanning the visible range to beyond the second near-infrared window

AU - Shapey, Jonathan

AU - Xie, Yijing

AU - Nabavi, Elham

AU - Ebner, Michael

AU - Saeed, Shakeel R

AU - Kitchen, Neil

AU - Dorward, Neil

AU - Grieve, Joan

AU - McEvoy, Andrew W

AU - Miserocchi, Anna

AU - Grover, Patrick

AU - Bradford, Robert

AU - Lim, Yau-Mun

AU - Ourselin, Sebastien

AU - Brandner, Sebastian

AU - Jaunmuktane, Zane

AU - Vercauteren, Tom

N1 - Funding Information: This study was supported by Wellcome Trust [203145Z/16/Z; 203148/Z/16/Z; WT106882], EPSRC [NS/A000050/1; NS/A000049/1] and National Brain Appeal [NBA/T&I/N-ONC] funding. Tom Vercauteren is supported by a Medtronic/Royal Academy of Engineering Research Chair [RCSRF1819\7\34]. Publisher Copyright: © 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.

PY - 2022/4

Y1 - 2022/4

N2 - Neuro-oncology surgery would benefit from detailed intraoperative tissue characterization provided by noncontact, contrast-agent-free, noninvasive optical imaging methods. In-depth knowledge of target tissue optical properties across a wide-wavelength spectrum could inform the design of optical imaging and computational methods to enable robust tissue analysis during surgery. We adapted a dual-beam integrating sphere to analyse small tissue samples and investigated ex vivo optical properties of five types of human brain tumour (meningioma, pituitary adenoma, schwannoma, low- and high-grade glioma) and nine different types of healthy brain tissue across a wavelength spectrum of 400 to 1800 nm. Fresh and frozen tissue samples were analysed. All tissue types demonstrated similar absorption spectra, but the reduced scattering coefficients of tumours show visible differences in the obtained optical spectrum compared to those of surrounding normal tissue. These results underline the potential of optical imaging technologies for intraoperative tissue characterization.

AB - Neuro-oncology surgery would benefit from detailed intraoperative tissue characterization provided by noncontact, contrast-agent-free, noninvasive optical imaging methods. In-depth knowledge of target tissue optical properties across a wide-wavelength spectrum could inform the design of optical imaging and computational methods to enable robust tissue analysis during surgery. We adapted a dual-beam integrating sphere to analyse small tissue samples and investigated ex vivo optical properties of five types of human brain tumour (meningioma, pituitary adenoma, schwannoma, low- and high-grade glioma) and nine different types of healthy brain tissue across a wavelength spectrum of 400 to 1800 nm. Fresh and frozen tissue samples were analysed. All tissue types demonstrated similar absorption spectra, but the reduced scattering coefficients of tumours show visible differences in the obtained optical spectrum compared to those of surrounding normal tissue. These results underline the potential of optical imaging technologies for intraoperative tissue characterization.

UR - http://www.scopus.com/inward/record.url?scp=85124527696&partnerID=8YFLogxK

U2 - 10.1002/jbio.202100072

DO - 10.1002/jbio.202100072

M3 - Article

C2 - 35048541

SN - 1864-063X

VL - 15

JO - Journal of biophotonics

JF - Journal of biophotonics

IS - 4

M1 - e202100072

ER -