Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications

Richard Taylor; Corinne Houart

doi:10.1007/978-1-0716-3401-1_7

Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications

Richard Taylor^*, Corinne Houart^*

^*Corresponding author for this work

Developmental Neurobiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Primary cell culture is an invaluable method frequently used to overcome challenges associated with in vivo experiments. In zebrafish research, in vivo live imaging experiments are routine owing to the high optical transparency of embryos, and, as a result, primary cell culture has been less utilized. However, the approach still boasts powerful advantages, emphasizing the importance of sophisticated zebrafish cell culture protocols. Here, we present an enhanced protocol for the generation of primary cell cultures by dissociation of 24 hpf zebrafish embryos. We include a novel cell culture medium recipe specifically favoring neuronal growth and survival, enabling relatively long-term culture. We outline primary zebrafish neuronal culture on glass coverslips, as well as in transwell inserts which allow isolation of neurite tissue for experiments such as investigating subcellular transcriptomes.

Original language	English
Title of host publication	Zebrafish: Methods in Molecular Biology
Publisher	SPRINGER
DOIs	https://doi.org/10.1007/978-1-0716-3401-1_7
Publication status	Published - 6 Sept 2023

Access to Document

10.1007/978-1-0716-3401-1_7

Cite this

@inbook{2e05ce1802dd4e63ae1b31aed0e05af0,

title = "Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications",

abstract = "Primary cell culture is an invaluable method frequently used to overcome challenges associated with in vivo experiments. In zebrafish research, in vivo live imaging experiments are routine owing to the high optical transparency of embryos, and, as a result, primary cell culture has been less utilized. However, the approach still boasts powerful advantages, emphasizing the importance of sophisticated zebrafish cell culture protocols. Here, we present an enhanced protocol for the generation of primary cell cultures by dissociation of 24 hpf zebrafish embryos. We include a novel cell culture medium recipe specifically favoring neuronal growth and survival, enabling relatively long-term culture. We outline primary zebrafish neuronal culture on glass coverslips, as well as in transwell inserts which allow isolation of neurite tissue for experiments such as investigating subcellular transcriptomes.",

author = "Richard Taylor and Corinne Houart",

year = "2023",

month = sep,

day = "6",

doi = "10.1007/978-1-0716-3401-1_7",

language = "English",

booktitle = "Zebrafish: Methods in Molecular Biology",

publisher = "SPRINGER",

}

TY - CHAP

T1 - Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications

AU - Taylor, Richard

AU - Houart, Corinne

PY - 2023/9/6

Y1 - 2023/9/6

N2 - Primary cell culture is an invaluable method frequently used to overcome challenges associated with in vivo experiments. In zebrafish research, in vivo live imaging experiments are routine owing to the high optical transparency of embryos, and, as a result, primary cell culture has been less utilized. However, the approach still boasts powerful advantages, emphasizing the importance of sophisticated zebrafish cell culture protocols. Here, we present an enhanced protocol for the generation of primary cell cultures by dissociation of 24 hpf zebrafish embryos. We include a novel cell culture medium recipe specifically favoring neuronal growth and survival, enabling relatively long-term culture. We outline primary zebrafish neuronal culture on glass coverslips, as well as in transwell inserts which allow isolation of neurite tissue for experiments such as investigating subcellular transcriptomes.

AB - Primary cell culture is an invaluable method frequently used to overcome challenges associated with in vivo experiments. In zebrafish research, in vivo live imaging experiments are routine owing to the high optical transparency of embryos, and, as a result, primary cell culture has been less utilized. However, the approach still boasts powerful advantages, emphasizing the importance of sophisticated zebrafish cell culture protocols. Here, we present an enhanced protocol for the generation of primary cell cultures by dissociation of 24 hpf zebrafish embryos. We include a novel cell culture medium recipe specifically favoring neuronal growth and survival, enabling relatively long-term culture. We outline primary zebrafish neuronal culture on glass coverslips, as well as in transwell inserts which allow isolation of neurite tissue for experiments such as investigating subcellular transcriptomes.

U2 - 10.1007/978-1-0716-3401-1_7

DO - 10.1007/978-1-0716-3401-1_7

M3 - Chapter

BT - Zebrafish: Methods in Molecular Biology

PB - SPRINGER

ER -

Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications

Abstract

Access to Document

Fingerprint

Cite this