Overview of the different methods used in the primary culture of oral mucosa cells

A Bryja; M Popis; B Borowiec; M Dyszkiewicz-Konwińska; I Kocherova; A Angelova-Volponi; K Mehr; M Bruska; M Nowicki; B Kempisty

Overview of the different methods used in the primary culture of oral mucosa cells

A Bryja, M Popis, B Borowiec, M Dyszkiewicz-Konwińska, I Kocherova, A Angelova-Volponi, K Mehr, M Bruska, M Nowicki, B Kempisty

Centre for Dental Education

Research output: Contribution to journal › Letter › peer-review

4 Citations (Scopus)

Abstract

The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.

Original language	English
Pages (from-to)	397-401
Number of pages	5
Journal	Journal of Biological Regulators and Homeostatic Agents
Volume	33
Issue number	2
Publication status	Published - 20 Mar 2019

Keywords

Direct explant method
Enzymatic method
Oral mucosa
Primary culture

Cite this

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abstract = "The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.",

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AU - Bryja, A

AU - Popis, M

AU - Borowiec, B

AU - Dyszkiewicz-Konwińska, M

AU - Kocherova, I

AU - Angelova-Volponi, A

AU - Mehr, K

AU - Bruska, M

AU - Nowicki, M

AU - Kempisty, B

PY - 2019/3/20

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N2 - The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.

AB - The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.

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KW - Enzymatic method

KW - Oral mucosa

KW - Primary culture

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M3 - Letter

C2 - 30887798

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Overview of the different methods used in the primary culture of oral mucosa cells

Abstract

Keywords

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