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Overview of the different methods used in the primary culture of oral mucosa cells

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Overview of the different methods used in the primary culture of oral mucosa cells. / Bryja, A; Popis, M; Borowiec, B; Dyszkiewicz-Konwińska, M; Kocherova, I; Angelova-Volponi, A; Mehr, K; Bruska, M; Nowicki, M; Kempisty, B.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 33, No. 2, 20.03.2019, p. 397-401.

Research output: Contribution to journalLetterpeer-review

Harvard

Bryja, A, Popis, M, Borowiec, B, Dyszkiewicz-Konwińska, M, Kocherova, I, Angelova-Volponi, A, Mehr, K, Bruska, M, Nowicki, M & Kempisty, B 2019, 'Overview of the different methods used in the primary culture of oral mucosa cells', Journal of Biological Regulators and Homeostatic Agents, vol. 33, no. 2, pp. 397-401.

APA

Bryja, A., Popis, M., Borowiec, B., Dyszkiewicz-Konwińska, M., Kocherova, I., Angelova-Volponi, A., Mehr, K., Bruska, M., Nowicki, M., & Kempisty, B. (2019). Overview of the different methods used in the primary culture of oral mucosa cells. Journal of Biological Regulators and Homeostatic Agents, 33(2), 397-401.

Vancouver

Bryja A, Popis M, Borowiec B, Dyszkiewicz-Konwińska M, Kocherova I, Angelova-Volponi A et al. Overview of the different methods used in the primary culture of oral mucosa cells. Journal of Biological Regulators and Homeostatic Agents. 2019 Mar 20;33(2):397-401.

Author

Bryja, A ; Popis, M ; Borowiec, B ; Dyszkiewicz-Konwińska, M ; Kocherova, I ; Angelova-Volponi, A ; Mehr, K ; Bruska, M ; Nowicki, M ; Kempisty, B. / Overview of the different methods used in the primary culture of oral mucosa cells. In: Journal of Biological Regulators and Homeostatic Agents. 2019 ; Vol. 33, No. 2. pp. 397-401.

Bibtex Download

@article{1d4085b3401f4a4bb69ce143283ba445,
title = "Overview of the different methods used in the primary culture of oral mucosa cells",
abstract = "The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.",
keywords = "Direct explant method, Enzymatic method, Oral mucosa, Primary culture",
author = "A Bryja and M Popis and B Borowiec and M Dyszkiewicz-Konwi{\'n}ska and I Kocherova and A Angelova-Volponi and K Mehr and M Bruska and M Nowicki and B Kempisty",
year = "2019",
month = mar,
day = "20",
language = "English",
volume = "33",
pages = "397--401",
journal = "Journal of Biological Regulators and Homeostatic Agents",
issn = "0393-974X",
publisher = "Biolife SAS",
number = "2",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Overview of the different methods used in the primary culture of oral mucosa cells

AU - Bryja, A

AU - Popis, M

AU - Borowiec, B

AU - Dyszkiewicz-Konwińska, M

AU - Kocherova, I

AU - Angelova-Volponi, A

AU - Mehr, K

AU - Bruska, M

AU - Nowicki, M

AU - Kempisty, B

PY - 2019/3/20

Y1 - 2019/3/20

N2 - The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.

AB - The culture of primary cells in vitro has enabled to gain knowledge in the field of cell biology, disease mechanisms and to offer great potential in drug testing. To date, two main techniques of isolating and culturing oral mucosal cells, the direct explant method and the enzymatic method, dominate the literature and practice. In the present study, both techniques are discussed in detail, comparing the advantages and disadvantages of the two approaches in setting up a primary culture of oral mucosal cell. The direct explant technique is well-established and has been commonly used for the past 20-30 years. Although the method of setting up the cultures did not show much variations in the methodology described by authors, the culturing conditions varied according to the aims of the projects.

KW - Direct explant method

KW - Enzymatic method

KW - Oral mucosa

KW - Primary culture

UR - http://www.scopus.com/inward/record.url?scp=85064723145&partnerID=8YFLogxK

M3 - Letter

C2 - 30887798

VL - 33

SP - 397

EP - 401

JO - Journal of Biological Regulators and Homeostatic Agents

JF - Journal of Biological Regulators and Homeostatic Agents

SN - 0393-974X

IS - 2

ER -

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