Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish: Food Chemistry

D.-G. Zhang; T. Zhao; C. Hogstrand; H.-M. Ye; X.-J. Xu; Z. Luo

doi:10.1016/j.foodchem.2021.129814

Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish: Food Chemistry

D.-G. Zhang, T. Zhao, C. Hogstrand, H.-M. Ye, X.-J. Xu, Z. Luo

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

At present, the harmful effects and relevant mechanism of oxidized fish oils on fish and fish cells remain unknown. Our study found that oxidized fish oils increased lipogenesis, and reduced lipolysis, activated oxidative stress by decreasing glutathione peroxidase (GPX) activity, increasing malondialdhyde (MDA) content and damaging mitochondrial structure, and activated autophagy in the liver of yellow catfish; oxidized eicosapentaenoic acid (oxEPA) induced oxidative stress in yellow catfish hepatocytes. Oxidative stress, mitochondrial dysfunction and lipophagy mediated oxEPA induced-variations in lipid metabolism. Our further investigation indicated that oxEPA-activated lipophagy was via inhibiting the DNA binding capacity of the cAMP-response element binding protein (CREB)-1 to the region of Bcl-2 promoter, which in turn suppressed the binding activity of Bcl-2 to Beclin1 and promoted autophagosome formation. For the first time, our study elucidated the mechanisms of oxidized fish oils-induced lipid deposition by the oxidative stress, mitochondrial dysfunction and CREB1-Bcl-2-Beclin1 pathway in fish. © 2021 Elsevier Ltd

Original language	English
Journal	Food Chem.
Volume	360
DOIs	https://doi.org/10.1016/j.foodchem.2021.129814
Publication status	Published - 2021

Keywords

Lipid metabolism
Molecular mechanism
Oxidative stress
Oxidized fish oils
Signaling pathway
Cell death
Deposition
Fish
Mitochondria
Oxidation
Unsaturated fatty acids
Eicosapentaenoic acid
Fish oil
Hepatocytes
Lipid deposition
Lipid metabolisms
Liver tissue
Mitochondrial dysfunction
Oxidized fish oil
Signalling pathways
Metabolism
3 methyladenine
acetylcysteine
beclin 1
chloroquine
cyclic AMP responsive element binding protein
fish oil
glutathione peroxidase
icosapentaenoic acid
malonaldehyde
protein bcl 2
rapamycin
reactive oxygen metabolite
lipid
animal cell
animal experiment
animal model
animal tissue
Article
autophagosome
autophagy (cellular)
binding affinity
controlled study
disorders of mitochondrial functions
DNA binding
enzyme activity
female
lipid metabolism
lipid storage
lipogenesis
lipolysis
lipophagocytosis
liver cell
liver tissue
male
nonalcoholic fatty liver
nonhuman
oxidative stress
Pelteobagrus fulvidraco
promoter region
protein content
signal transduction
animal
autophagy
catfish
cell culture
drug effect
liver
metabolism
mitochondrion
oxidation reduction reaction
Animals
Autophagy
Beclin-1
Catfishes
Cells, Cultured
Cyclic AMP Response Element-Binding Protein
Fish Oils
Lipid Metabolism
Lipids
Liver
Oxidation-Reduction
Oxidative Stress
Proto-Oncogene Proteins c-bcl-2
Signal Transduction

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10.1016/j.foodchem.2021.129814

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107706688&doi=10.1016%2fj.foodchem.2021.129814&partnerID=40&md5=7eb68c62f644083639d0b368fd59dfa0

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title = "Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish: Food Chemistry",

abstract = "At present, the harmful effects and relevant mechanism of oxidized fish oils on fish and fish cells remain unknown. Our study found that oxidized fish oils increased lipogenesis, and reduced lipolysis, activated oxidative stress by decreasing glutathione peroxidase (GPX) activity, increasing malondialdhyde (MDA) content and damaging mitochondrial structure, and activated autophagy in the liver of yellow catfish; oxidized eicosapentaenoic acid (oxEPA) induced oxidative stress in yellow catfish hepatocytes. Oxidative stress, mitochondrial dysfunction and lipophagy mediated oxEPA induced-variations in lipid metabolism. Our further investigation indicated that oxEPA-activated lipophagy was via inhibiting the DNA binding capacity of the cAMP-response element binding protein (CREB)-1 to the region of Bcl-2 promoter, which in turn suppressed the binding activity of Bcl-2 to Beclin1 and promoted autophagosome formation. For the first time, our study elucidated the mechanisms of oxidized fish oils-induced lipid deposition by the oxidative stress, mitochondrial dysfunction and CREB1-Bcl-2-Beclin1 pathway in fish. {\textcopyright} 2021 Elsevier Ltd",

keywords = "Lipid metabolism, Molecular mechanism, Oxidative stress, Oxidized fish oils, Signaling pathway, Cell death, Deposition, Fish, Mitochondria, Oxidation, Unsaturated fatty acids, Eicosapentaenoic acid, Fish oil, Hepatocytes, Lipid deposition, Lipid metabolisms, Liver tissue, Mitochondrial dysfunction, Oxidized fish oil, Signalling pathways, Metabolism, 3 methyladenine, acetylcysteine, beclin 1, chloroquine, cyclic AMP responsive element binding protein, fish oil, glutathione peroxidase, icosapentaenoic acid, malonaldehyde, protein bcl 2, rapamycin, reactive oxygen metabolite, lipid, animal cell, animal experiment, animal model, animal tissue, Article, autophagosome, autophagy (cellular), binding affinity, controlled study, disorders of mitochondrial functions, DNA binding, enzyme activity, female, lipid metabolism, lipid storage, lipogenesis, lipolysis, lipophagocytosis, liver cell, liver tissue, male, nonalcoholic fatty liver, nonhuman, oxidative stress, Pelteobagrus fulvidraco, promoter region, protein content, signal transduction, animal, autophagy, catfish, cell culture, drug effect, liver, metabolism, mitochondrion, oxidation reduction reaction, Animals, Autophagy, Beclin-1, Catfishes, Cells, Cultured, Cyclic AMP Response Element-Binding Protein, Fish Oils, Lipid Metabolism, Lipids, Liver, Oxidation-Reduction, Oxidative Stress, Proto-Oncogene Proteins c-bcl-2, Signal Transduction",

author = "D.-G. Zhang and T. Zhao and C. Hogstrand and H.-M. Ye and X.-J. Xu and Z. Luo",

note = "Cited By :38 Export Date: 19 July 2024 CODEN: FOCHD Correspondence Address: Luo, Z.; Laboratory of Molecular Nutrition, China; email: [email protected] Chemicals/CAS: 3 methyladenine, 5142-23-4; acetylcysteine, 616-91-1; chloroquine, 132-73-0, 3545-67-3, 50-63-5, 54-05-7; cyclic AMP responsive element binding protein, 130428-87-4, 130939-96-7; fish oil, 308064-99-5, 8016-13-5; glutathione peroxidase, 9013-66-5; icosapentaenoic acid, 10417-94-4, 1553-41-9, 25378-27-2, 32839-30-8; malonaldehyde, 542-78-9; protein bcl 2, 219306-68-0; rapamycin, 53123-88-9; lipid, 66455-18-3; Beclin-1; Cyclic AMP Response Element-Binding Protein; Fish Oils; Lipids; Proto-Oncogene Proteins c-bcl-2 Manufacturers: MedChemexpress, United States; MilliporeSigma, United States; Selleck, United States Funding details: National Natural Science Foundation of China, NSFC, 31372547 Funding details: National Key Research and Development Program of China, NKRDPC, 2018YFD0900400 Funding text 1: The study is funded by National Key R&D Program of China (grant no. 2018YFD0900400) and National Natural Science Foundation of China (NSFC, grant no. 31372547)",

year = "2021",

doi = "10.1016/j.foodchem.2021.129814",

language = "English",

volume = "360",

journal = "Food Chem.",

issn = "0308-8146",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Oxidized fish oils increased lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the CREB1-Bcl2-Beclin1 pathway in the liver tissues and hepatocytes of yellow catfish

T2 - Food Chemistry

AU - Zhang, D.-G.

AU - Zhao, T.

AU - Hogstrand, C.

AU - Ye, H.-M.

AU - Xu, X.-J.

AU - Luo, Z.

N1 - Cited By :38 Export Date: 19 July 2024 CODEN: FOCHD Correspondence Address: Luo, Z.; Laboratory of Molecular Nutrition, China; email: [email protected] Chemicals/CAS: 3 methyladenine, 5142-23-4; acetylcysteine, 616-91-1; chloroquine, 132-73-0, 3545-67-3, 50-63-5, 54-05-7; cyclic AMP responsive element binding protein, 130428-87-4, 130939-96-7; fish oil, 308064-99-5, 8016-13-5; glutathione peroxidase, 9013-66-5; icosapentaenoic acid, 10417-94-4, 1553-41-9, 25378-27-2, 32839-30-8; malonaldehyde, 542-78-9; protein bcl 2, 219306-68-0; rapamycin, 53123-88-9; lipid, 66455-18-3; Beclin-1; Cyclic AMP Response Element-Binding Protein; Fish Oils; Lipids; Proto-Oncogene Proteins c-bcl-2 Manufacturers: MedChemexpress, United States; MilliporeSigma, United States; Selleck, United States Funding details: National Natural Science Foundation of China, NSFC, 31372547 Funding details: National Key Research and Development Program of China, NKRDPC, 2018YFD0900400 Funding text 1: The study is funded by National Key R&D Program of China (grant no. 2018YFD0900400) and National Natural Science Foundation of China (NSFC, grant no. 31372547)

PY - 2021

Y1 - 2021

N2 - At present, the harmful effects and relevant mechanism of oxidized fish oils on fish and fish cells remain unknown. Our study found that oxidized fish oils increased lipogenesis, and reduced lipolysis, activated oxidative stress by decreasing glutathione peroxidase (GPX) activity, increasing malondialdhyde (MDA) content and damaging mitochondrial structure, and activated autophagy in the liver of yellow catfish; oxidized eicosapentaenoic acid (oxEPA) induced oxidative stress in yellow catfish hepatocytes. Oxidative stress, mitochondrial dysfunction and lipophagy mediated oxEPA induced-variations in lipid metabolism. Our further investigation indicated that oxEPA-activated lipophagy was via inhibiting the DNA binding capacity of the cAMP-response element binding protein (CREB)-1 to the region of Bcl-2 promoter, which in turn suppressed the binding activity of Bcl-2 to Beclin1 and promoted autophagosome formation. For the first time, our study elucidated the mechanisms of oxidized fish oils-induced lipid deposition by the oxidative stress, mitochondrial dysfunction and CREB1-Bcl-2-Beclin1 pathway in fish. © 2021 Elsevier Ltd

AB - At present, the harmful effects and relevant mechanism of oxidized fish oils on fish and fish cells remain unknown. Our study found that oxidized fish oils increased lipogenesis, and reduced lipolysis, activated oxidative stress by decreasing glutathione peroxidase (GPX) activity, increasing malondialdhyde (MDA) content and damaging mitochondrial structure, and activated autophagy in the liver of yellow catfish; oxidized eicosapentaenoic acid (oxEPA) induced oxidative stress in yellow catfish hepatocytes. Oxidative stress, mitochondrial dysfunction and lipophagy mediated oxEPA induced-variations in lipid metabolism. Our further investigation indicated that oxEPA-activated lipophagy was via inhibiting the DNA binding capacity of the cAMP-response element binding protein (CREB)-1 to the region of Bcl-2 promoter, which in turn suppressed the binding activity of Bcl-2 to Beclin1 and promoted autophagosome formation. For the first time, our study elucidated the mechanisms of oxidized fish oils-induced lipid deposition by the oxidative stress, mitochondrial dysfunction and CREB1-Bcl-2-Beclin1 pathway in fish. © 2021 Elsevier Ltd

KW - Lipid metabolism

KW - Molecular mechanism

KW - Oxidative stress

KW - Oxidized fish oils

KW - Signaling pathway

KW - Cell death

KW - Deposition

KW - Fish

KW - Mitochondria

KW - Oxidation

KW - Unsaturated fatty acids

KW - Eicosapentaenoic acid

KW - Fish oil

KW - Hepatocytes

KW - Lipid deposition

KW - Lipid metabolisms

KW - Liver tissue

KW - Mitochondrial dysfunction

KW - Oxidized fish oil

KW - Signalling pathways

KW - Metabolism

KW - 3 methyladenine

KW - acetylcysteine

KW - beclin 1

KW - chloroquine

KW - cyclic AMP responsive element binding protein

KW - fish oil

KW - glutathione peroxidase

KW - icosapentaenoic acid

KW - malonaldehyde

KW - protein bcl 2

KW - rapamycin

KW - reactive oxygen metabolite

KW - lipid

KW - animal cell

KW - animal experiment

KW - animal model

KW - animal tissue

KW - Article

KW - autophagosome

KW - autophagy (cellular)

KW - binding affinity

KW - controlled study

KW - disorders of mitochondrial functions

KW - DNA binding

KW - enzyme activity

KW - female

KW - lipid metabolism

KW - lipid storage

KW - lipogenesis

KW - lipolysis

KW - lipophagocytosis

KW - liver cell

KW - liver tissue

KW - male

KW - nonalcoholic fatty liver

KW - nonhuman

KW - oxidative stress

KW - Pelteobagrus fulvidraco

KW - promoter region

KW - protein content

KW - signal transduction

KW - animal

KW - autophagy

KW - catfish

KW - cell culture

KW - drug effect

KW - liver

KW - metabolism

KW - mitochondrion

KW - oxidation reduction reaction

KW - Animals

KW - Autophagy

KW - Beclin-1

KW - Catfishes

KW - Cells, Cultured

KW - Cyclic AMP Response Element-Binding Protein

KW - Fish Oils

KW - Lipid Metabolism

KW - Lipids

KW - Liver

KW - Oxidation-Reduction

KW - Oxidative Stress

KW - Proto-Oncogene Proteins c-bcl-2

KW - Signal Transduction

U2 - 10.1016/j.foodchem.2021.129814

DO - 10.1016/j.foodchem.2021.129814

M3 - Article

SN - 0308-8146

VL - 360

JO - Food Chem.

JF - Food Chem.

ER -