p120 catenin associates with microtubules - Inverse relationship between microtubule binding and Rho GTPase regulation

TY - JOUR

T1 - p120 catenin associates with microtubules - Inverse relationship between microtubule binding and Rho GTPase regulation

AU - Franz, C M

AU - Ridley, A J

PY - 2004/2/20

Y1 - 2004/2/20

N2 - p120 catenin (p120ctn), an armadillo protein and component of the cadherin adhesion complex, has been found recently to induce a dendritic morphology by regulating Rho family GTPases. We have identified specific serines within the Arm repeat domain that, when mutated to alanine, promote p120ctn association with interphase microtubules, leading to microtubule reorganization and stabilization. The mutant p120ctn also localized to the mitotic spindle and centrosomes. In contrast to wild-type p120ctn, the microtubule-associated p120ctn mutant did not activate Rac1 and did not induce a dendritic morphology. In addition, we show that a basic motif within the p120ctn Arm repeat domain known to be required for the inhibition of RhoA is also required for binding to microtubules. We therefore propose that binding of p120ctn to microtubules is inversely related to its ability to regulate Rho GTPases

AB - p120 catenin (p120ctn), an armadillo protein and component of the cadherin adhesion complex, has been found recently to induce a dendritic morphology by regulating Rho family GTPases. We have identified specific serines within the Arm repeat domain that, when mutated to alanine, promote p120ctn association with interphase microtubules, leading to microtubule reorganization and stabilization. The mutant p120ctn also localized to the mitotic spindle and centrosomes. In contrast to wild-type p120ctn, the microtubule-associated p120ctn mutant did not activate Rac1 and did not induce a dendritic morphology. In addition, we show that a basic motif within the p120ctn Arm repeat domain known to be required for the inhibition of RhoA is also required for binding to microtubules. We therefore propose that binding of p120ctn to microtubules is inversely related to its ability to regulate Rho GTPases

U2 - 10.1074/jbc.M312812200

DO - 10.1074/jbc.M312812200

M3 - Article

SN - 1083-351X

VL - 279

SP - 6588

EP - 6594

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

ER -