TY - JOUR
T1 - Patterns of subgingival microbiota in different periodontal phenotypes
AU - Nibali, L.
AU - Sousa, V.
AU - Davrandi, M.
AU - Liu, L. S.
AU - Spratt, D.
AU - Donos, N.
N1 - Funding Information:
We acknowledge the Oral theme of the NIHR UCLH/UCL Biomedical Research Centre (BRC) for this work and the study took place within the Eastman Clinical Investigation Centre (ECIC), which also has funding from the Biomedical Research Centre (BRC). The help of Dr. Anna Tymon, Dr. Kruti Patel and Prof. Steve Humphries for the laboratory analyses and of Drs Ulpee Darbar, Jeanie Suvan and Thanasak Rakmanee for the clinical visits are gratefully acknowledged.
Publisher Copyright:
© 2021
PY - 2022/2
Y1 - 2022/2
N2 - Objectives: To compare the subgingival microbiota of patients with aggressive (AgP) or chronic periodontitis (CP) to healthy (H), non-periodontitis patients as well as to explore their relevant associations to different host genetic variants. Methods: Following clinical examination, blood and subgingival plaque sampling of 471 study participants (125 AgP, 121 CP, 225 H), subgingival community analysis was performed by next generation sequencing of the 16S rRNA. Microbial data from 266 participants (75 AgP, 95 CP, 98 H) were available for analysis. SNPs in the IL6, IL6R and FTO gene were selected for genetic marker analyses. Results: Combined periodontitis patients (AgP + CP), particularly those classified with AgP, exhibited lower alpha- and beta- diversity. Several genera (including Peptostreptococcaceae, Filifactor, Desulfobulbus, Tannerella and Lachnospiracee) and species were over-abundant in combined periodontitis vs. healthy individuals, while other genera such as Prevotella or Dialister were found to be more abundant in healthy cases. The only genus with difference in abundance between AgP and CP was Granulicatella. No associations between IL6, IL6RA and FTO genetic variants and microbial findings were detected. Conclusion: This study suggests that limited microbial differences existed between AgP and CP and challenges the current notion that periodontitis is associated with increased subgingival microbial diversity compared with periodontal health. Clinical significance: The findings of this study cast some doubts on the notion that the dysbiosis characteristic of periodontal disease is expressed as increased microbial diversity.
AB - Objectives: To compare the subgingival microbiota of patients with aggressive (AgP) or chronic periodontitis (CP) to healthy (H), non-periodontitis patients as well as to explore their relevant associations to different host genetic variants. Methods: Following clinical examination, blood and subgingival plaque sampling of 471 study participants (125 AgP, 121 CP, 225 H), subgingival community analysis was performed by next generation sequencing of the 16S rRNA. Microbial data from 266 participants (75 AgP, 95 CP, 98 H) were available for analysis. SNPs in the IL6, IL6R and FTO gene were selected for genetic marker analyses. Results: Combined periodontitis patients (AgP + CP), particularly those classified with AgP, exhibited lower alpha- and beta- diversity. Several genera (including Peptostreptococcaceae, Filifactor, Desulfobulbus, Tannerella and Lachnospiracee) and species were over-abundant in combined periodontitis vs. healthy individuals, while other genera such as Prevotella or Dialister were found to be more abundant in healthy cases. The only genus with difference in abundance between AgP and CP was Granulicatella. No associations between IL6, IL6RA and FTO genetic variants and microbial findings were detected. Conclusion: This study suggests that limited microbial differences existed between AgP and CP and challenges the current notion that periodontitis is associated with increased subgingival microbial diversity compared with periodontal health. Clinical significance: The findings of this study cast some doubts on the notion that the dysbiosis characteristic of periodontal disease is expressed as increased microbial diversity.
KW - Aggressive periodontitis
KW - Chronic periodontitis
KW - Genetics
KW - Microbiota
KW - Periodontitis
UR - http://www.scopus.com/inward/record.url?scp=85121262765&partnerID=8YFLogxK
U2 - 10.1016/j.jdent.2021.103912
DO - 10.1016/j.jdent.2021.103912
M3 - Article
AN - SCOPUS:85121262765
SN - 0300-5712
VL - 117
JO - Journal of Dentistry
JF - Journal of Dentistry
M1 - 103912
ER -