PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

Pornjira Somnark; Natcha Chyerochana; Akechai Kongprajug; Skorn Mongkolsuk; Kwanrawee Sirikanchana

doi:10.1016/j.dib.2018.04.129

PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

Pornjira Somnark, Natcha Chyerochana, Akechai Kongprajug, Skorn Mongkolsuk, Kwanrawee Sirikanchana^*

^*Corresponding author for this work

Randall Centre of Cell & Molecular Biophysics

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA Bacteroidales genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100–110.

Original language	English
Pages (from-to)	156-169
Number of pages	14
Journal	Data in Brief
Volume	19
DOIs	https://doi.org/10.1016/j.dib.2018.04.129
Publication status	Published - Aug 2018

Keywords

Bacteroidales
Endpoint PCR
Fecal pollution
Microbial source tracking
Sensitivity
Specificity
Water quality

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.dib.2018.04.129

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title = "PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers",

abstract = "We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA Bacteroidales genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100–110.",

keywords = "Bacteroidales, Endpoint PCR, Fecal pollution, Microbial source tracking, Sensitivity, Specificity, Water quality",

author = "Pornjira Somnark and Natcha Chyerochana and Akechai Kongprajug and Skorn Mongkolsuk and Kwanrawee Sirikanchana",

note = "Funding Information: This research was financially supported by the Thailand Research Fund (Contract no. SRI5930305 ) and the Kurita Water and Environmental Foundation (KWEF) – Asian Institute of Technology (AIT) research grant. Publisher Copyright: {\textcopyright} 2018 The Authors",

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doi = "10.1016/j.dib.2018.04.129",

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AU - Somnark, Pornjira

AU - Chyerochana, Natcha

AU - Kongprajug, Akechai

AU - Mongkolsuk, Skorn

AU - Sirikanchana, Kwanrawee

N1 - Funding Information: This research was financially supported by the Thailand Research Fund (Contract no. SRI5930305 ) and the Kurita Water and Environmental Foundation (KWEF) – Asian Institute of Technology (AIT) research grant. Publisher Copyright: © 2018 The Authors

PY - 2018/8

Y1 - 2018/8

N2 - We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA Bacteroidales genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100–110.

AB - We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA Bacteroidales genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of Bacteroidales genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100–110.

KW - Bacteroidales

KW - Endpoint PCR

KW - Fecal pollution

KW - Microbial source tracking

KW - Sensitivity

KW - Specificity

KW - Water quality

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DO - 10.1016/j.dib.2018.04.129

M3 - Article

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SN - 2352-3409

VL - 19

SP - 156

EP - 169

JO - Data in Brief

JF - Data in Brief

ER -

PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

Abstract

Keywords

UN SDGs

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