Peptidomic changes of saliva after non-surgical treatment of stage I/II generalized periodontitis

TY - JOUR

T1 - Peptidomic changes of saliva after non-surgical treatment of stage I/II generalized periodontitis

AU - Yuan, Chao

AU - Ma, Zhangke

AU - Tong, Peiyuan

AU - Yu, Shunlan

AU - Li, Yi

AU - Gallagher, Jennifer Elizabeth

AU - Sun, Xiangyu

AU - Zheng, Shuguo

N1 - Funding Information: The authors appreciate financial support from the National Natural Science Foundation of China (grant number: 81801037), the Beijing Municipal Science and Technology Commission (grant number: Z181100001618015), and the Ministry of Science and Technology of the People's Republic of China (grant number: 2018FY101005). We also appreciate the Joint International Research Center of Translational and Clinical Research between Peking University Health Science Center and King's College London. Publisher Copyright: © 2021 Wiley Periodicals LLC Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3/19

Y1 - 2021/3/19

N2 - Objective: To explore the changes of peptidome profiles of saliva, serum, and gingival crevicular fluid (GCF) before and after non-surgical periodontal treatment in patients with generalized periodontitis (stage I/II). Subjects and methods: Saliva, serum, and GCF samples were collected from 17 patients at baseline (T0), one week after ultrasonic supragingival scaling (T1) and eight weeks after subgingival scaling and root planning (T2). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was carried out to detect changes in peptidomic profiles. Then, nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC/ESI-MS/MS) was performed to identify potential peptide biomarkers. Results: Most of the peptides from the patients exhibited a decreasing trend from the time point of pretreatment to that of post-treatment. Cluster analysis and scatter plots using these peptides indicated that salivary peptidome has an acceptable capability of reflecting the status of stage I/II generalized periodontitis. Seven of these peptides were successfully identified as α-1-antitrypsin, immunoglobulin κ variable 4-1, haptoglobin, and immunoglobulin heavy constant γ2. Conclusions: Certain peptides in saliva, serum, and GCF were down-regulated after non-surgical periodontal treatment, demonstrating the application prospects of saliva in monitoring and surveillance of periodontal diseases in both clinical settings and communities.

AB - Objective: To explore the changes of peptidome profiles of saliva, serum, and gingival crevicular fluid (GCF) before and after non-surgical periodontal treatment in patients with generalized periodontitis (stage I/II). Subjects and methods: Saliva, serum, and GCF samples were collected from 17 patients at baseline (T0), one week after ultrasonic supragingival scaling (T1) and eight weeks after subgingival scaling and root planning (T2). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was carried out to detect changes in peptidomic profiles. Then, nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC/ESI-MS/MS) was performed to identify potential peptide biomarkers. Results: Most of the peptides from the patients exhibited a decreasing trend from the time point of pretreatment to that of post-treatment. Cluster analysis and scatter plots using these peptides indicated that salivary peptidome has an acceptable capability of reflecting the status of stage I/II generalized periodontitis. Seven of these peptides were successfully identified as α-1-antitrypsin, immunoglobulin κ variable 4-1, haptoglobin, and immunoglobulin heavy constant γ2. Conclusions: Certain peptides in saliva, serum, and GCF were down-regulated after non-surgical periodontal treatment, demonstrating the application prospects of saliva in monitoring and surveillance of periodontal diseases in both clinical settings and communities.

KW - chronic periodontitis

KW - mass spectrometry

KW - non-surgical treatment

KW - saliva

UR - http://www.scopus.com/inward/record.url?scp=85102696784&partnerID=8YFLogxK

U2 - 10.1111/odi.13838

DO - 10.1111/odi.13838

M3 - Article

AN - SCOPUS:85102696784

SN - 1354-523X

JO - Oral Diseases

JF - Oral Diseases

ER -