Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo

Doris Proebstl; Mathieu-Benoit Voisin; Abigail Woodfin; James Whiteford; Fulvio D'Acquisto; Gareth E. Jones; David Rowe; Sussan Nourshargh

doi:10.1084/jem.20111622

Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo

Doris Proebstl, Mathieu-Benoit Voisin, Abigail Woodfin, James Whiteford, Fulvio D'Acquisto, Gareth E. Jones, David Rowe, Sussan Nourshargh

Research output: Contribution to journal › Article › peer-review

355 Citations (Scopus)

Abstract

Neutrophil transmigration through venular walls that are composed of endothelial cells (ECs), pericytes, and the venular basement membrane is a key component of innate immunity. Through direct analysis of leukocyte-pericyte interactions in inflamed tissues using confocal intravital microscopy, we show how pericytes facilitate transmigration in vivo. After EC migration, neutrophils crawl along pericyte processes to gaps between adjacent pericytes in an ICAM-1-, Mac-1-, and LFA-1-dependent manner. These gaps were enlarged in inflamed tissues through pericyte shape change and were used as exit points by neutrophils in breaching the venular wall. The findings identify previously unknown roles for pericytes in neutrophil transmigration in vivo and add additional steps to the leukocyte adhesion cascade that supports leukocyte trafficking into sites of inflammation.

Original language	English
Pages (from-to)	1219-1234
Number of pages	16
Journal	Journal of Experimental Medicine
Volume	209
Issue number	6
DOIs	https://doi.org/10.1084/jem.20111622
Publication status	Published - 4 Jun 2012

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title = "Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo",

abstract = "Neutrophil transmigration through venular walls that are composed of endothelial cells (ECs), pericytes, and the venular basement membrane is a key component of innate immunity. Through direct analysis of leukocyte-pericyte interactions in inflamed tissues using confocal intravital microscopy, we show how pericytes facilitate transmigration in vivo. After EC migration, neutrophils crawl along pericyte processes to gaps between adjacent pericytes in an ICAM-1-, Mac-1-, and LFA-1-dependent manner. These gaps were enlarged in inflamed tissues through pericyte shape change and were used as exit points by neutrophils in breaching the venular wall. The findings identify previously unknown roles for pericytes in neutrophil transmigration in vivo and add additional steps to the leukocyte adhesion cascade that supports leukocyte trafficking into sites of inflammation.",

author = "Doris Proebstl and Mathieu-Benoit Voisin and Abigail Woodfin and James Whiteford and Fulvio D'Acquisto and Jones, {Gareth E.} and David Rowe and Sussan Nourshargh",

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doi = "10.1084/jem.20111622",

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T1 - Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo

AU - Proebstl, Doris

AU - Voisin, Mathieu-Benoit

AU - Woodfin, Abigail

AU - Whiteford, James

AU - D'Acquisto, Fulvio

AU - Jones, Gareth E.

AU - Rowe, David

AU - Nourshargh, Sussan

PY - 2012/6/4

Y1 - 2012/6/4

N2 - Neutrophil transmigration through venular walls that are composed of endothelial cells (ECs), pericytes, and the venular basement membrane is a key component of innate immunity. Through direct analysis of leukocyte-pericyte interactions in inflamed tissues using confocal intravital microscopy, we show how pericytes facilitate transmigration in vivo. After EC migration, neutrophils crawl along pericyte processes to gaps between adjacent pericytes in an ICAM-1-, Mac-1-, and LFA-1-dependent manner. These gaps were enlarged in inflamed tissues through pericyte shape change and were used as exit points by neutrophils in breaching the venular wall. The findings identify previously unknown roles for pericytes in neutrophil transmigration in vivo and add additional steps to the leukocyte adhesion cascade that supports leukocyte trafficking into sites of inflammation.

AB - Neutrophil transmigration through venular walls that are composed of endothelial cells (ECs), pericytes, and the venular basement membrane is a key component of innate immunity. Through direct analysis of leukocyte-pericyte interactions in inflamed tissues using confocal intravital microscopy, we show how pericytes facilitate transmigration in vivo. After EC migration, neutrophils crawl along pericyte processes to gaps between adjacent pericytes in an ICAM-1-, Mac-1-, and LFA-1-dependent manner. These gaps were enlarged in inflamed tissues through pericyte shape change and were used as exit points by neutrophils in breaching the venular wall. The findings identify previously unknown roles for pericytes in neutrophil transmigration in vivo and add additional steps to the leukocyte adhesion cascade that supports leukocyte trafficking into sites of inflammation.

U2 - 10.1084/jem.20111622

DO - 10.1084/jem.20111622

M3 - Article

SN - 0022-1007

VL - 209

SP - 1219

EP - 1234

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 6

ER -

Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo

Abstract

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