Research output: Contribution to journal › Article › peer-review
Douglas M Lopes, Franziska Denk, Kim I Chisholm, Tesha Suddason, Camille Durrieux, Matthew Thakur, Clive Gentry, Stephen B McMahon
Original language | English |
---|---|
Pages (from-to) | 1314-1322 |
Journal | Pain |
Volume | 158 |
Issue number | 7 |
DOIs | |
Accepted/In press | 27 Mar 2017 |
Published | Jul 2017 |
Peripheral inflammatory pain sensitisation_LOPES_PublishedJuly2017_GOLD VoR (CC BY)
Peripheral_inflammatory_pain_sensitisation_LOPES_PublishedJuly2017_GOLD_VoR_CC_BY_.pdf, 590 KB, application/pdf
Uploaded date:10 Jul 2017
Version:Final published version
Licence:CC BY
The immune and sensory systems are known for their close proximity and interaction. Indeed, in a variety of pain states a myriad of different immune cells are activated and recruited, playing a key role in neuronal sensitisation. During inflammatory pain it is thought that mast cells (MC) are one of the immune cell types involved in this process, but so far the evidence outlining their direct effect on neuronal cells remains unclear. To clarify whether MC are involved in inflammatory pain states, we used a transgenic mouse line (Mctp5CreiDTR) in which MC could be depleted in an inducible manner by administration of diphtheria toxin. Our results show that ablation of MC in male mice did not result in any change in mechanical and thermal hypersensitivity in the CFA model of inflammatory pain. Similarly, edema and temperature triggered by CFA inflammation at the injection site remained identical in MC depleted mice compared to their littermate controls. Additionally, we show that Mctp5Cre-iDTR mice display normal levels of mechanical hypersensitivity after local injection of NGF, a factor well characterised to produce peripheral sensitisation and for being upregulated upon injury and inflammation. We also demonstrate that NGF treatment in vitro does not lead to an increased level of TNFα in bone marrow-derived MC. Furthermore, our qRT-PCR data reveal that MC express negligible levels of NGF receptors, thereby explaining the lack of response to NGF. Together, our data suggest that MC do not play a direct role in peripheral sensitisation during inflammatory conditions.
King's College London - Homepage
© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454