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Peripheral phenotype and gene expression profiles of combined liver-kidney transplant patients

Research output: Contribution to journalArticle

Erwan Dumontet ; Richard Danger ; Parsia A. Vagefi ; Maria Carlota Londoño ; Annaïck Pallier ; Juan José Lozano ; Magali Giral ; Nicolas Degauque ; Jean Paul Soulillou ; Marc Martínez-Llordella ; Herman Lee ; Marianne Latournerie ; Karim Boudjema ; Joelle Dulong ; Karin Tarte ; Alberto Sanchez-Fueyo ; Sandy Feng ; Sophie Brouard ; Sophie Conchon

Original languageEnglish
Pages (from-to)401–409
Number of pages9
JournalLIVER INTERNATIONAL
Volume36
Issue number3
Early online date8 Aug 2015
DOIs
StatePublished - Mar 2016

King's Authors

Abstract

Background and Aims

The beneficial effect of one graft on another has been reported in combined transplantation but the associated mechanisms and biological influence of each graft have not yet been established.

Methods

In multiple analyses, we explored the PBMC phenotype and signature of 45 immune-related messenger RNAs and 754 microRNAs from a total of 235 patients, including combined liver–kidney transplant recipients (CLK), patients with a liver (L-STA) or kidney (K-STA) graft only under classical immunosuppression and patients with tolerated liver (L-TOL) or kidney grafts (K-TOL).

Results

CLK show an intermediary phenotype with a higher percentage of peripheral CD19+CD24+CD38Low memory B cells and Helios+ Treg cells, two features associated with tolerance profiles, compared to L-STA and K-STA (P < 0.05, P < 0.01). Very few miRNA were significantly differentially expressed in CLK vs. K-STA and even fewer when compared to L-STA (35 and 8, P < 0.05). Finally, CLK are predicted to share common miRNA targets with K-TOL and even more with L-TOL (344 and 411, P = 0.005). Altogether CLK display an intermediary phenotype and gene profile, which is closer to that of liver transplant patients, with possible similarities with the profiles of tolerant patients.

Conclusion

These data suggest that CLK patients show the immunological influence of both allografts with liver having a greater influence.

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