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Peroxidasin-like protein: a novel peroxidase homologue in the human heart

Research output: Contribution to journalArticle

Zalan Peterfi, Zsuzsanna E. Toth, Hajnal A. Kovacs, Eniko Lazar, Adrienn Sum, Agnes Donko, Gabor Sirokmany, Ajay M. Shah, Miklos Geiszt

Original languageEnglish
Article numberN/A
Pages (from-to)393-399
Number of pages7
JournalCardiovascular Research
Volume101
Issue number3
DOIs
Publication statusPublished - 1 Mar 2014

King's Authors

Abstract

Aims: Peroxidases serve diverse biological functions including well-characterized activities in host defence and hormone biosynthesis. More recently, peroxidasin (PXDN) was found to be involved in collagen IV cross-linking in the extracellular matrix (ECM). The aim of this study was to characterize the expression and function of peroxidasin-like protein (PXDNL), a previously unknown peroxidase homologue.

Methods and results: We cloned the PXDNL cDNA from the human heart and identified its expression pattern by northern blot, in situ hybridization, and immunohistochemistry. PXDNL is expressed exclusively in the heart and it has evolved to lose its peroxidase activity. The protein is produced by cardiomyocytes and localizes to cell-cell junctions. We also demonstrate that PXDNL can form a complex with PXDN and antagonizes its peroxidase activity. Furthermore, we show an increased expression of PXDNL in the failing myocardium.

Conclusion: PXDNL is a unique component of the heart with a recently evolved inactivation of peroxidase function. The elevation of PXDNL levels in the failing heart may contribute to ECM dysregulation due to its antagonism of PXDN function.

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