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Photon counting imaging and centroiding with an electron-bombarded CCD using single molecule localisation software

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)121-125
JournalNuclear Instruments & Methods In Physics Research Section A, Accelerators, Spectrometers, Detectors And Associated Equipment
Volume820
Early online date10 Mar 2016
DOIs
Publication statusPublished - 1 Jun 2016

Documents

  • 2016_Hirvonen_NimA

    2016_Hirvonen_NimA.pdf, 1.61 MB, application/pdf

    9/05/2016

    Final published version

    CC BY

King's Authors

Abstract

Photon event centroiding in photon counting imaging and single-molecule localisation in super-resolution fluorescence microscopy share many traits. Although photon event centroiding has traditionally been performed with simple single-iteration algorithms, we recently reported that iterative fitting algorithms originally developed for single-molecule localisation fluorescence microscopy work very well when applied to centroiding photon events imaged with an MCP-intensified CMOS camera. Here, we have applied these algorithms for centroiding of photon events from an electron-bombarded CCD (EBCCD). We find that centroiding algorithms based on iterative fitting of the photon events yield excellent results and allow fitting of overlapping photon events, a feature not reported before and an important aspect to facilitate an increased count rate and shorter acquisition times.

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