Photon counting phosphorescence lifetime imaging with TimepixCam

Liisa Maija Hirvonen, Merlin Fisher-Levine, Klaus Suhling, Andrei Nomerotski

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)
212 Downloads (Pure)

Abstract

TimepixCam is a novel fast optical imager based on an optimized silicon pixel sensor with a thin entrance window and read out by a Timepix Application Specific Integrated Circuit. The 256 × 256 pixel sensor has a time resolution of 15 ns at a sustained frame rate of 10 Hz. We used this sensor in combination with an image intensifier for wide-field time-correlated single photon counting imaging. We have characterised the photon detection capabilities of this detector system and employed it on a wide-field epifluorescence microscope to map phosphorescence decays of various iridium complexes with lifetimes of about 1 μs in 200 μm diameter polystyrene beads.

Original languageEnglish
Article number013104
Number of pages6
JournalREVIEW OF SCIENTIFIC INSTRUMENTS
Volume88
Issue number1
Early online dateJan 2017
DOIs
Publication statusPublished - 1 Jan 2017

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