TY - JOUR
T1 - Pitfalls in the characterization of circulating and tissue-resident human γδ T cells
AU - Beucke, Nicola
AU - Wesch, Daniela
AU - Oberg, Hans-Heinrich
AU - Peters, Christian
AU - Bochem, Jonas
AU - Weide, Benjamin
AU - Garbe, Claus
AU - Pawelec, Graham
AU - Sebens, Susanne
AU - Röcken, Christoph
AU - Hashimoto, Hisayoshi
AU - Löffler, Markus W
AU - Nocerino, Paola
AU - Kordasti, Shahram
AU - Kabelitz, Dieter
AU - Schilbach, Karin
AU - Wistuba-Hamprecht, Kilian
N1 - ©2020 Society for Leukocyte Biology.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Dissection of the role and function of human γδ T cells and their heterogeneous subsets in cancer, inflammation, and auto-immune diseases is a growing and dynamic research field of increasing interest to the scientific community. Therefore, harmonization and standardization of techniques for the characterization of peripheral and tissue-resident γδ T cells is crucial to facilitate comparability between published and emerging research. The application of commercially available reagents to classify γδ T cells, in particular the combination of multiple Abs, is not always trouble-free, posing major demands on researchers entering this field. Occasionally, even entire γδ T cell subsets may remain undetected when certain Abs are combined in flow cytometric analysis with multicolor Ab panels, or might be lost during cell isolation procedures. Here, based on the recent literature and our own experience, we provide an overview of methods commonly employed for the phenotypic and functional characterization of human γδ T cells including advanced polychromatic flow cytometry, mass cytometry, immunohistochemistry, and magnetic cell isolation. We highlight potential pitfalls and discuss how to circumvent these obstacles.
AB - Dissection of the role and function of human γδ T cells and their heterogeneous subsets in cancer, inflammation, and auto-immune diseases is a growing and dynamic research field of increasing interest to the scientific community. Therefore, harmonization and standardization of techniques for the characterization of peripheral and tissue-resident γδ T cells is crucial to facilitate comparability between published and emerging research. The application of commercially available reagents to classify γδ T cells, in particular the combination of multiple Abs, is not always trouble-free, posing major demands on researchers entering this field. Occasionally, even entire γδ T cell subsets may remain undetected when certain Abs are combined in flow cytometric analysis with multicolor Ab panels, or might be lost during cell isolation procedures. Here, based on the recent literature and our own experience, we provide an overview of methods commonly employed for the phenotypic and functional characterization of human γδ T cells including advanced polychromatic flow cytometry, mass cytometry, immunohistochemistry, and magnetic cell isolation. We highlight potential pitfalls and discuss how to circumvent these obstacles.
KW - flow cytometry
KW - immunohistochemistry
KW - immunomonitoring
KW - magnetic cell isolation
KW - mass cytometry
KW - γδ T cells
UR - http://www.scopus.com/inward/record.url?scp=85078675873&partnerID=8YFLogxK
U2 - 10.1002/JLB.5MA1219-296R
DO - 10.1002/JLB.5MA1219-296R
M3 - Article
C2 - 31967358
SN - 0741-5400
VL - 107
SP - 1097
EP - 1105
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 6
ER -
