TY - JOUR
T1 - PKA regulatory subunit expression in tooth development
AU - de Sousa, Sílvia Ferreira
AU - Kawasaki, Katsushige
AU - Kawasaki, Maiko
AU - Volponi, Ana Angelova
AU - Gomez, Ricardo Santiago
AU - Gomes, Carolina Cavaliéri
AU - Sharpe, Paul T
AU - Ohazama, Atsushi
N1 - Copyright © 2014 Elsevier B.V. All rights reserved.
PY - 2014/5
Y1 - 2014/5
N2 - Protein kinase A (PK A) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present study was to assess the expression of alpha isoforms of PICA regulatory subunit (Prkar1a and Prkar2a) in mouse and human odontogenesis by in situ hybridization. PRKAR1A and PRKAR2A mRNA transcription was further confirmed in a human deciduous germ by qRT-PCR. Mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis. These isoforms thus showed different expression pattern between human and mouse tooth germs.
AB - Protein kinase A (PK A) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present study was to assess the expression of alpha isoforms of PICA regulatory subunit (Prkar1a and Prkar2a) in mouse and human odontogenesis by in situ hybridization. PRKAR1A and PRKAR2A mRNA transcription was further confirmed in a human deciduous germ by qRT-PCR. Mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis. These isoforms thus showed different expression pattern between human and mouse tooth germs.
KW - Animals
KW - Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
KW - Gene Expression Regulation, Developmental
KW - Humans
KW - In Situ Hybridization
KW - Mice
KW - Odontogenesis
KW - RNA Isoforms
KW - Tooth, Deciduous
U2 - 10.1016/j.gep.2014.04.002
DO - 10.1016/j.gep.2014.04.002
M3 - Article
C2 - 24755349
SN - 1567-133X
VL - 15
SP - 46
EP - 51
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 1
ER -