Planar Airy beam light-sheet for two-photon microscopy

Neveen A Hosny, James A Seyforth, Gunnar Spickermann, Thomas J Mitchell, Pedro Almada, Robert Chesters, Scott J Mitchell, George Chennell, Anthony C Vernon, Kwangwook Cho, Deepak P Srivastava, Robert Forster, Tom Vettenburg

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.

Original languageEnglish
Pages (from-to)3927-3935
Number of pages9
JournalBiomedical Optics Express
Volume11
Issue number7
DOIs
Publication statusPublished - 1 Jul 2020

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