@article{7d9122d9d25a489ba7e61e70bb8f5fbb,
title = "Polarized illumination coded structured illumination microscopy (picoSIM): Experimental results",
abstract = "The need for acquiring at least three images to reconstruct an optical section of a sample limits the acquisition rate in structured illumination microscopy (SIM) for optical sectioning. In polarized illumination coded structured illumination microscopy (picoSIM) the three individual light patterns are encoded in a single polarized illumination light distribution, enabling the acquisition of the complete SIM data in a single exposure. Here, we describe our experimental set-up and show experimental results acquired with sequential and single-shot picoSIM. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'. ",
keywords = "fluorescence, resolution, sectioning, structured illumination, super-resolution",
author = "Daniel Appelt and Elisabeth Ehler and {Shukla Mukherjee}, Sapna and Rainer Heintzmann and Kai Wicker",
note = "Funding Information: We are further thankful to Jan Modersitzki to provide the FAIR image registration toolbox. S.S. was funded by the Deutsche Forschungsgemeinschaft under the grant no. HE-3492-3.2. Funding Information: This work was funded by the British Heart Foundation (BHF) under grant no. RE/08/003. The fluorescent plane sample was generously provided by G. J. Brakenhoff, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands. The image splitter designed by Rainer Pick, was purchased from Stefan Hell, Max-Planck-Institut f{\"u}r biophysikalische Chemie, G{\"o}ttingen, Germany. Acknowledgements Publisher Copyright: {\textcopyright} 2022 Royal Society Publishing. All rights reserved.",
year = "2022",
doi = "10.1098/rsta.2021.0193",
language = "English",
volume = "380",
journal = "Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences",
issn = "1364-503X",
publisher = "The Royal Society",
number = "2220",
}