Polarized illumination coded structured illumination microscopy (picoSIM): Experimental results

Daniel Appelt, Elisabeth Ehler, Sapna Shukla Mukherjee, Rainer Heintzmann*, Kai Wicker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The need for acquiring at least three images to reconstruct an optical section of a sample limits the acquisition rate in structured illumination microscopy (SIM) for optical sectioning. In polarized illumination coded structured illumination microscopy (picoSIM) the three individual light patterns are encoded in a single polarized illumination light distribution, enabling the acquisition of the complete SIM data in a single exposure. Here, we describe our experimental set-up and show experimental results acquired with sequential and single-shot picoSIM. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.

Original languageEnglish
Article number20210193
JournalPhilosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
Issue number2220
Publication statusPublished - 2022


  • fluorescence
  • resolution
  • sectioning
  • structured illumination
  • super-resolution


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