Polarized illumination coded structured illumination microscopy (picoSIM): Experimental results

TY - JOUR

T1 - Polarized illumination coded structured illumination microscopy (picoSIM)

T2 - Experimental results

AU - Appelt, Daniel

AU - Ehler, Elisabeth

AU - Shukla Mukherjee, Sapna

AU - Heintzmann, Rainer

AU - Wicker, Kai

N1 - Funding Information: We are further thankful to Jan Modersitzki to provide the FAIR image registration toolbox. S.S. was funded by the Deutsche Forschungsgemeinschaft under the grant no. HE-3492-3.2. Funding Information: This work was funded by the British Heart Foundation (BHF) under grant no. RE/08/003. The fluorescent plane sample was generously provided by G. J. Brakenhoff, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands. The image splitter designed by Rainer Pick, was purchased from Stefan Hell, Max-Planck-Institut für biophysikalische Chemie, Göttingen, Germany. Acknowledgements Publisher Copyright: © 2022 Royal Society Publishing. All rights reserved.

PY - 2022

Y1 - 2022

N2 - The need for acquiring at least three images to reconstruct an optical section of a sample limits the acquisition rate in structured illumination microscopy (SIM) for optical sectioning. In polarized illumination coded structured illumination microscopy (picoSIM) the three individual light patterns are encoded in a single polarized illumination light distribution, enabling the acquisition of the complete SIM data in a single exposure. Here, we describe our experimental set-up and show experimental results acquired with sequential and single-shot picoSIM. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.

AB - The need for acquiring at least three images to reconstruct an optical section of a sample limits the acquisition rate in structured illumination microscopy (SIM) for optical sectioning. In polarized illumination coded structured illumination microscopy (picoSIM) the three individual light patterns are encoded in a single polarized illumination light distribution, enabling the acquisition of the complete SIM data in a single exposure. Here, we describe our experimental set-up and show experimental results acquired with sequential and single-shot picoSIM. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.

KW - fluorescence

KW - resolution

KW - sectioning

KW - structured illumination

KW - super-resolution

UR - https://www.scopus.com/pages/publications/85124636633

U2 - 10.1098/rsta.2021.0193

DO - 10.1098/rsta.2021.0193

M3 - Article

C2 - 35152759

AN - SCOPUS:85124636633

SN - 1364-503X

VL - 380

JO - Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

JF - Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

IS - 2220

M1 - 20210193

ER -