Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells

Katja Gehmlich, Katrin Hayess, Christof Legler, Sophie Haebel, Peter F. M. Van der Ven, Elisabeth Ehler, Dieter O. Fuerst

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Skeletal muscle differentiation is a complex process: It is characterised by changes in gene expression and protein composition. Simultaneously, a dramatic remodelling of the cytoskeleton and associated cell-matrix contacts, the costameres, occurs. The expression and localisation of the protein ponsin at cell-matrix contacts marks the establishment of costameres. In this report we show that skeletal muscle cells are characterised by a novel ponsin isoform, which contains a large insertion in its carboxy-terminus. This skeletal muscle-specific module binds the adapter proteins Nck1 and Nck2, and increased co-localisation of ponsin with Nck2 is observed at remodelling cell-matrix contacts of differentiating skeletal muscle cells. Since this ponsin insertion can be phosphorylated, it may adjust the interaction affinity with Nck adapter proteins. The novel ponsin isoform and its interaction with Nck1/2 provide exciting insight into the convergence of signalling pathways at the costameres, and its crucial role for skeletal muscle differentiation and re-generation. (c) 2010 Elsevier GmbH. All rights reserved.
Original languageEnglish
Pages (from-to)351 - 364
Number of pages14
JournalEuropean Journal of Cell Biology
Volume89
Issue number5
DOIs
Publication statusPublished - May 2010

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