Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS

Fabian Hellbach; Lucy Sinke; Ricardo Costeira; Sebastian-Edgar Baumeister; Marian Beekman; Panayiotis Louca; Emily r. Leeming; Olatz Mompeo; Sarah Berry; Rory Wilson; Nina Wawro; Dennis Freuer; Hans Hauner; Annette Peters; Juliane Winkelmann; Wolfgang Koenig; Christa Meisinger; Melanie Waldenberger; Bastiaan t. Heijmans; P. eline Slagboom; Jordana t. Bell; Jakob Linseisen

doi:10.1007/s00394-022-03074-9

Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS

Fabian Hellbach, Lucy Sinke, Ricardo Costeira, Sebastian-Edgar Baumeister, Marian Beekman, Panayiotis Louca, Emily r. Leeming, Olatz Mompeo, Sarah Berry, Rory Wilson, Nina Wawro, Dennis Freuer, Hans Hauner, Annette Peters, Juliane Winkelmann, Wolfgang Koenig, Christa Meisinger, Melanie Waldenberger, Bastiaan t. Heijmans, P. eline SlagboomJordana t. Bell, Jakob Linseisen

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Purpose: Examining epigenetic patterns is a crucial step in identifying molecular changes of disease pathophysiology, with DNA methylation as the most accessible epigenetic measure. Diet is suggested to affect metabolism and health via epigenetic modifications. Thus, our aim was to explore the association between food consumption and DNA methylation. Methods: Epigenome-wide association studies were conducted in three cohorts: KORA FF4, TwinsUK, and Leiden Longevity Study, and 37 dietary exposures were evaluated. Food group definition was harmonized across the three cohorts. DNA methylation was measured using Infinium MethylationEPIC BeadChip in KORA and Infinium HumanMethylation450 BeadChip in the Leiden study and the TwinsUK study. Overall, data from 2293 middle-aged men and women were included. A fixed-effects meta-analysis pooled study-specific estimates. The significance threshold was set at 0.05 for false-discovery rate-adjusted p values per food group. Results: We identified significant associations between the methylation level of CpG sites and the consumption of onions and garlic (2), nuts and seeds (18), milk (1), cream (11), plant oils (4), butter (13), and alcoholic beverages (27). The signals targeted genes of metabolic health relevance, for example, GLI1, RPTOR, and DIO1, among others. Conclusion: This EWAS is unique with its focus on food groups that are part of a Western diet. Significant findings were mostly related to food groups with a high-fat content.

Original language	English
Pages (from-to)	1357-1375
Number of pages	19
Journal	EUROPEAN JOURNAL OF NUTRITION
Volume	62
Issue number	3
Early online date	26 Dec 2022
DOIs	https://doi.org/10.1007/s00394-022-03074-9
Publication status	Published - 1 Apr 2023

Access to Document

10.1007/s00394-022-03074-9Licence: CC BY

Cite this

Hellbach, F., Sinke, L., Costeira, R., Baumeister, S.-E., Beekman, M., Louca, P., Leeming, E. R., Mompeo, O., Berry, S., Wilson, R., Wawro, N., Freuer, D., Hauner, H., Peters, A., Winkelmann, J., Koenig, W., Meisinger, C., Waldenberger, M., Heijmans, B. T., ... Linseisen, J. (2023). Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS. EUROPEAN JOURNAL OF NUTRITION, 62(3), 1357-1375. https://doi.org/10.1007/s00394-022-03074-9

@article{e33107096d464368a013360d5331c02f,

title = "Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS",

abstract = "Purpose: Examining epigenetic patterns is a crucial step in identifying molecular changes of disease pathophysiology, with DNA methylation as the most accessible epigenetic measure. Diet is suggested to affect metabolism and health via epigenetic modifications. Thus, our aim was to explore the association between food consumption and DNA methylation. Methods: Epigenome-wide association studies were conducted in three cohorts: KORA FF4, TwinsUK, and Leiden Longevity Study, and 37 dietary exposures were evaluated. Food group definition was harmonized across the three cohorts. DNA methylation was measured using Infinium MethylationEPIC BeadChip in KORA and Infinium HumanMethylation450 BeadChip in the Leiden study and the TwinsUK study. Overall, data from 2293 middle-aged men and women were included. A fixed-effects meta-analysis pooled study-specific estimates. The significance threshold was set at 0.05 for false-discovery rate-adjusted p values per food group. Results: We identified significant associations between the methylation level of CpG sites and the consumption of onions and garlic (2), nuts and seeds (18), milk (1), cream (11), plant oils (4), butter (13), and alcoholic beverages (27). The signals targeted genes of metabolic health relevance, for example, GLI1, RPTOR, and DIO1, among others. Conclusion: This EWAS is unique with its focus on food groups that are part of a Western diet. Significant findings were mostly related to food groups with a high-fat content.",

author = "Fabian Hellbach and Lucy Sinke and Ricardo Costeira and Sebastian-Edgar Baumeister and Marian Beekman and Panayiotis Louca and Leeming, {Emily r.} and Olatz Mompeo and Sarah Berry and Rory Wilson and Nina Wawro and Dennis Freuer and Hans Hauner and Annette Peters and Juliane Winkelmann and Wolfgang Koenig and Christa Meisinger and Melanie Waldenberger and Heijmans, {Bastiaan t.} and Slagboom, {P. eline} and Bell, {Jordana t.} and Jakob Linseisen",

note = "Funding Information: Open Access funding enabled and organized by Projekt DEAL. Dietary assessment in KORA FF4 was supported by iMED, a research alliance within the Helmholtz Association, Germany. The KORA study was initiated and financed by the Helmholtz Zentrum M{\"u}nchen–German Research Center for Environmental Health, which is funded by the BMBF and by the State of Bavaria. Furthermore, KORA research was supported within the Munich Center of Health Sciences (MC-Health), Ludwig-Maximilians- Universit{\"a}t, as part of LMUinnovativ. The funding agencies had no role in the design, analysis or writing of this article. The project DIMENSION got financial support by a grant of European HDHL Joint Programming Initiative funding scheme: Grant No.: 01EA1902B (F.H., J.L.). Additional work was supported by the Joint Programming Initiative {\textquoteleft}a Healthy Diet for a Healthy Life{\textquoteright} (JPI-HDHL) DIMENSION project [ZonMW project number: 529051021]. (L.S., B.T.H., E.S.) The funding agencies had no role in the design, analysis or writing of this article. The research leading to these results has received funding from the European Union{\textquoteright}s Seventh Framework Program (FP7/2007-2011) under grant agreement number 259679. This study was financially supported by the Innovation-Oriented Research Program on Genomics (SenterNovem IGE05007), the Centre for Medical Systems Biology and the Netherlands Consortium for Healthy Ageing (grant 050-060-810), all in the framework of the Netherlands Genomics Initiative, Netherlands Organization for Scientific Research (NWO), by Unilever Colworth and by BBMRI-NL, a Research Infrastructure financed by the Dutch government (NWO 184.021.007 and 184.033.111). (L.S., B.T.H., E.S.). The TwinsUK study is funded by the Wellcome Trust, Medical Research Council, Versus Arthritis, European Union Horizon 2020, Chronic Disease Research Foundation (CDRF), Zoe Global Ltd and the National Institute for Health Research (NIHR) Clinical Research Network (CRN) and Biomedical Research Centre based at Guy{\textquoteright}s and St Thomas{\textquoteright} NHS Foundation Trust in partnership with King{\textquoteright}s College London. R.C., S.B., and J.T.B. are supported by the European HDHL Joint Programming Initiative funding scheme DIMENSION project (BBSRC BB/S020845/1 and BB/T019980/1 to J.T.B.) Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2023",

month = apr,

day = "1",

doi = "10.1007/s00394-022-03074-9",

language = "English",

volume = "62",

pages = "1357--1375",

journal = "EUROPEAN JOURNAL OF NUTRITION",

issn = "1436-6207",

publisher = "D. Steinkopff-Verlag",

number = "3",

}

Hellbach, F, Sinke, L, Costeira, R, Baumeister, S-E, Beekman, M, Louca, P , Leeming, ER, Mompeo, O, Berry, S, Wilson, R, Wawro, N, Freuer, D, Hauner, H, Peters, A, Winkelmann, J, Koenig, W, Meisinger, C, Waldenberger, M, Heijmans, BT, Slagboom, PE, Bell, JT & Linseisen, J 2023, 'Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS', EUROPEAN JOURNAL OF NUTRITION, vol. 62, no. 3, pp. 1357-1375. https://doi.org/10.1007/s00394-022-03074-9

TY - JOUR

T1 - Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS

AU - Hellbach, Fabian

AU - Sinke, Lucy

AU - Costeira, Ricardo

AU - Baumeister, Sebastian-Edgar

AU - Beekman, Marian

AU - Louca, Panayiotis

AU - Leeming, Emily r.

AU - Mompeo, Olatz

AU - Berry, Sarah

AU - Wilson, Rory

AU - Wawro, Nina

AU - Freuer, Dennis

AU - Hauner, Hans

AU - Peters, Annette

AU - Winkelmann, Juliane

AU - Koenig, Wolfgang

AU - Meisinger, Christa

AU - Waldenberger, Melanie

AU - Heijmans, Bastiaan t.

AU - Slagboom, P. eline

AU - Bell, Jordana t.

AU - Linseisen, Jakob

N1 - Funding Information: Open Access funding enabled and organized by Projekt DEAL. Dietary assessment in KORA FF4 was supported by iMED, a research alliance within the Helmholtz Association, Germany. The KORA study was initiated and financed by the Helmholtz Zentrum München–German Research Center for Environmental Health, which is funded by the BMBF and by the State of Bavaria. Furthermore, KORA research was supported within the Munich Center of Health Sciences (MC-Health), Ludwig-Maximilians- Universität, as part of LMUinnovativ. The funding agencies had no role in the design, analysis or writing of this article. The project DIMENSION got financial support by a grant of European HDHL Joint Programming Initiative funding scheme: Grant No.: 01EA1902B (F.H., J.L.). Additional work was supported by the Joint Programming Initiative ‘a Healthy Diet for a Healthy Life’ (JPI-HDHL) DIMENSION project [ZonMW project number: 529051021]. (L.S., B.T.H., E.S.) The funding agencies had no role in the design, analysis or writing of this article. The research leading to these results has received funding from the European Union’s Seventh Framework Program (FP7/2007-2011) under grant agreement number 259679. This study was financially supported by the Innovation-Oriented Research Program on Genomics (SenterNovem IGE05007), the Centre for Medical Systems Biology and the Netherlands Consortium for Healthy Ageing (grant 050-060-810), all in the framework of the Netherlands Genomics Initiative, Netherlands Organization for Scientific Research (NWO), by Unilever Colworth and by BBMRI-NL, a Research Infrastructure financed by the Dutch government (NWO 184.021.007 and 184.033.111). (L.S., B.T.H., E.S.). The TwinsUK study is funded by the Wellcome Trust, Medical Research Council, Versus Arthritis, European Union Horizon 2020, Chronic Disease Research Foundation (CDRF), Zoe Global Ltd and the National Institute for Health Research (NIHR) Clinical Research Network (CRN) and Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust in partnership with King’s College London. R.C., S.B., and J.T.B. are supported by the European HDHL Joint Programming Initiative funding scheme DIMENSION project (BBSRC BB/S020845/1 and BB/T019980/1 to J.T.B.) Publisher Copyright: © 2022, The Author(s).

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Purpose: Examining epigenetic patterns is a crucial step in identifying molecular changes of disease pathophysiology, with DNA methylation as the most accessible epigenetic measure. Diet is suggested to affect metabolism and health via epigenetic modifications. Thus, our aim was to explore the association between food consumption and DNA methylation. Methods: Epigenome-wide association studies were conducted in three cohorts: KORA FF4, TwinsUK, and Leiden Longevity Study, and 37 dietary exposures were evaluated. Food group definition was harmonized across the three cohorts. DNA methylation was measured using Infinium MethylationEPIC BeadChip in KORA and Infinium HumanMethylation450 BeadChip in the Leiden study and the TwinsUK study. Overall, data from 2293 middle-aged men and women were included. A fixed-effects meta-analysis pooled study-specific estimates. The significance threshold was set at 0.05 for false-discovery rate-adjusted p values per food group. Results: We identified significant associations between the methylation level of CpG sites and the consumption of onions and garlic (2), nuts and seeds (18), milk (1), cream (11), plant oils (4), butter (13), and alcoholic beverages (27). The signals targeted genes of metabolic health relevance, for example, GLI1, RPTOR, and DIO1, among others. Conclusion: This EWAS is unique with its focus on food groups that are part of a Western diet. Significant findings were mostly related to food groups with a high-fat content.

AB - Purpose: Examining epigenetic patterns is a crucial step in identifying molecular changes of disease pathophysiology, with DNA methylation as the most accessible epigenetic measure. Diet is suggested to affect metabolism and health via epigenetic modifications. Thus, our aim was to explore the association between food consumption and DNA methylation. Methods: Epigenome-wide association studies were conducted in three cohorts: KORA FF4, TwinsUK, and Leiden Longevity Study, and 37 dietary exposures were evaluated. Food group definition was harmonized across the three cohorts. DNA methylation was measured using Infinium MethylationEPIC BeadChip in KORA and Infinium HumanMethylation450 BeadChip in the Leiden study and the TwinsUK study. Overall, data from 2293 middle-aged men and women were included. A fixed-effects meta-analysis pooled study-specific estimates. The significance threshold was set at 0.05 for false-discovery rate-adjusted p values per food group. Results: We identified significant associations between the methylation level of CpG sites and the consumption of onions and garlic (2), nuts and seeds (18), milk (1), cream (11), plant oils (4), butter (13), and alcoholic beverages (27). The signals targeted genes of metabolic health relevance, for example, GLI1, RPTOR, and DIO1, among others. Conclusion: This EWAS is unique with its focus on food groups that are part of a Western diet. Significant findings were mostly related to food groups with a high-fat content.

UR - http://www.scopus.com/inward/record.url?scp=85144895926&partnerID=8YFLogxK

U2 - 10.1007/s00394-022-03074-9

DO - 10.1007/s00394-022-03074-9

M3 - Article

SN - 1436-6207

VL - 62

SP - 1357

EP - 1375

JO - EUROPEAN JOURNAL OF NUTRITION

JF - EUROPEAN JOURNAL OF NUTRITION

IS - 3

ER -

Pooled analysis of epigenome-wide association studies of food consumption in KORA, TwinsUK and LLS

Abstract

Access to Document

Other files and links

Fingerprint

Cite this