TY - JOUR
T1 - Potential anticancer effect of carvacrol codrugs on human glioblastoma cells
AU - Yazici, Ayşenur
AU - Marinelli, Lisa
AU - Cacciatore, Ivana
AU - Emsen, Bugrahan
AU - Eusepi, Piera
AU - Di Biase, Giuseppe
AU - Di Stefano, Antonio
AU - Mardinoğlu, Adil
AU - Türkez, Hasan
N1 - Funding Information:
The authors declare no conflict of interest. This work was partly supported by the Italian Ministry of Education University and Research (FAR 2018) and Erzurum Technical University (BAP-2018).
Publisher Copyright:
© 2021 Bentham Science Publishers.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Background: Essential oils are considered as promising sources of novel anticancer compounds. Carvacrol (CVC), the major constituent of many aromatic plants including oregano and thymus, is endowed with curative properties on different cancers, including liver, colon, and lung. Little information is available regarding the potential of CVC for the treatment of brain cancers, notably glioblastoma multiforme (GBM). Objective: In this work, we investigated the in vitro effect of CVC codrugs (CVC1-8), synthesized by direct-coupled co-drug strategies, on human glioblastoma cell line (U87-MG) for the first time. Method: Cell viability was detected by MTT and LDH assays while expression levels of important genes (such as EGFR, NFKB1A, AKT1, AKT2, and others) associated with GBM and inflammatory pathway were detected by PCR array. Results: Results showed that CVC1-8 codrugs induced cytotoxicity and positive alterations in molecular responses on U87MG cells. Particularly, important pathways (such as PI3K/PTEN/AKT) involved in the onset and progression of GBM resulted in modulation by CVC3 and CVC8. Conclusion: Our results suggest that CVC3 and CVC8 could be suitable candidates for further investigation to develop new strategies for the prevention and/or treatment of GBM.
AB - Background: Essential oils are considered as promising sources of novel anticancer compounds. Carvacrol (CVC), the major constituent of many aromatic plants including oregano and thymus, is endowed with curative properties on different cancers, including liver, colon, and lung. Little information is available regarding the potential of CVC for the treatment of brain cancers, notably glioblastoma multiforme (GBM). Objective: In this work, we investigated the in vitro effect of CVC codrugs (CVC1-8), synthesized by direct-coupled co-drug strategies, on human glioblastoma cell line (U87-MG) for the first time. Method: Cell viability was detected by MTT and LDH assays while expression levels of important genes (such as EGFR, NFKB1A, AKT1, AKT2, and others) associated with GBM and inflammatory pathway were detected by PCR array. Results: Results showed that CVC1-8 codrugs induced cytotoxicity and positive alterations in molecular responses on U87MG cells. Particularly, important pathways (such as PI3K/PTEN/AKT) involved in the onset and progression of GBM resulted in modulation by CVC3 and CVC8. Conclusion: Our results suggest that CVC3 and CVC8 could be suitable candidates for further investigation to develop new strategies for the prevention and/or treatment of GBM.
KW - Anti-proliferative action
KW - Carvacrol codrug
KW - Codrug strategy
KW - Gene expression
KW - Glioblastoma multiforme
UR - http://www.scopus.com/inward/record.url?scp=85100244915&partnerID=8YFLogxK
U2 - 10.2174/1567201817666201027123424
DO - 10.2174/1567201817666201027123424
M3 - Article
C2 - 33109049
AN - SCOPUS:85100244915
SN - 1567-2018
VL - 18
SP - 349
EP - 355
JO - Current Drug Delivery
JF - Current Drug Delivery
IS - 3
ER -
