Pre-TCR signaling regulates IL-7 receptor alpha expression promoting thymocyte survival at the transition from the double-negative to double-positive stage

TY - JOUR

T1 - Pre-TCR signaling regulates IL-7 receptor alpha expression promoting thymocyte survival at the transition from the double-negative to double-positive stage

AU - Trigueros, C

AU - Hozumi, K

AU - Silva-Santos, B

AU - Bruno, L

AU - Hayday, A C

AU - Owen, M

AU - Pennington, D J

PY - 2003/7/1

Y1 - 2003/7/1

N2 - The pre-T cell receptor (pre-TCR) and IL-7 receptor (IL-7R) are critical mediators of survival, proliferation and differentiation in immature thymocytes. Here we show that pre-TCR signaling directly maintains IL-7alpha expression as developing thymocytes undergo beta-selection. Inhibition of IL-7/IL-7R signaling in (CD44(-)CD25(-)) DN4 cells results in decreased generation of double-positive thymocytes due to increased death of rapidly proliferating P-selected cells. Thus, we identify a mechanism by which pre-TCR signaling controls the selective survival of TCRbeta(+) thymocytes, and define a further stage of T cell differentiation in which signaling from a TCR regulates the ability of that cell to respond to cytokine.

AB - The pre-T cell receptor (pre-TCR) and IL-7 receptor (IL-7R) are critical mediators of survival, proliferation and differentiation in immature thymocytes. Here we show that pre-TCR signaling directly maintains IL-7alpha expression as developing thymocytes undergo beta-selection. Inhibition of IL-7/IL-7R signaling in (CD44(-)CD25(-)) DN4 cells results in decreased generation of double-positive thymocytes due to increased death of rapidly proliferating P-selected cells. Thus, we identify a mechanism by which pre-TCR signaling controls the selective survival of TCRbeta(+) thymocytes, and define a further stage of T cell differentiation in which signaling from a TCR regulates the ability of that cell to respond to cytokine.

UR - https://www.scopus.com/pages/publications/0042969051

U2 - 10.1002/eji.200323831

DO - 10.1002/eji.200323831

M3 - Article

VL - 33

SP - 1968

EP - 1977

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 7

ER -