Precautions in the use of 110MAG as a tracer of silver metabolism in ecotoxicology: Preferential bioconcentration of 109CD by trout gills after 110MAG exposure

Heinz J.M. Hansen; Martin Grosell; Ulf Jacobsen; Jesper C. Jørgensen; Christer Hogstrand; Christopher M. Wood

doi:10.1897/1551-5028(2002)021<1004:PITUOA>2.0.CO;2

Precautions in the use of ^110MAG as a tracer of silver metabolism in ecotoxicology: Preferential bioconcentration of ¹⁰⁹CD by trout gills after ^110MAG exposure

Heinz J.M. Hansen, Martin Grosell^*, Ulf Jacobsen, Jesper C. Jørgensen, Christer Hogstrand, Christopher M. Wood

^*Corresponding author for this work

King's College London

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

An often overlooked problem in the use of radiotracers is the possibility of isotopic contamination. Commercially available silver ^110M-Ag was used to study silver uptake and depuration in rainbow trout and European eel. Quality control by means of comparative γ and β counting brought our attention to a contamination of the ^110MAg stock with ¹⁰⁹Cd, which could be seen only because the ¹⁰⁹Cd was markedly bioconcentrated by trout gills. The contamination could not be detected in eel gills or in other tissues of both species. The difference between trout and eel gill structure and function is the probable explanation for the marked difference in ¹⁰⁹Cd accumulation. This contamination was identified as ¹⁰⁹Cd by γ spectroscopy and its origin by transmutation of natural silver as a result of neutron activation is described. Failure to recognize this contamination problem would have resulted in serious misinterpretation of the data set. Guidance for avoiding this problem is given.

Original language	English
Pages (from-to)	1004-1008
Number of pages	5
Journal	ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
Volume	21
Issue number	5
DOIs	https://doi.org/10.1897/1551-5028(2002)021<1004:PITUOA>2.0.CO;2
Publication status	Published - 1 May 2002

Keywords

Cadium
European eel
Rainbow trout
Silver
Subcellular distribution

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1897/1551-5028(2002)021<1004:PITUOA>2.0.CO;2

Cite this

Hansen, H. J. M., Grosell, M., Jacobsen, U., Jørgensen, J. C., Hogstrand, C., & Wood, C. M. (2002). Precautions in the use of ^110MAG as a tracer of silver metabolism in ecotoxicology: Preferential bioconcentration of ¹⁰⁹CD by trout gills after ^110MAG exposure. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 21(5), 1004-1008. https://doi.org/10.1897/1551-5028(2002)021<1004:PITUOA>2.0.CO;2

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title = "Precautions in the use of 110MAG as a tracer of silver metabolism in ecotoxicology: Preferential bioconcentration of 109CD by trout gills after 110MAG exposure",

abstract = "An often overlooked problem in the use of radiotracers is the possibility of isotopic contamination. Commercially available silver 110M-Ag was used to study silver uptake and depuration in rainbow trout and European eel. Quality control by means of comparative γ and β counting brought our attention to a contamination of the 110MAg stock with 109Cd, which could be seen only because the 109Cd was markedly bioconcentrated by trout gills. The contamination could not be detected in eel gills or in other tissues of both species. The difference between trout and eel gill structure and function is the probable explanation for the marked difference in 109Cd accumulation. This contamination was identified as 109Cd by γ spectroscopy and its origin by transmutation of natural silver as a result of neutron activation is described. Failure to recognize this contamination problem would have resulted in serious misinterpretation of the data set. Guidance for avoiding this problem is given.",

keywords = "Cadium, European eel, Rainbow trout, Silver, Subcellular distribution",

author = "Hansen, {Heinz J.M.} and Martin Grosell and Ulf Jacobsen and J{\o}rgensen, {Jesper C.} and Christer Hogstrand and Wood, {Christopher M.}",

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Hansen, HJM, Grosell, M, Jacobsen, U, Jørgensen, JC, Hogstrand, C & Wood, CM 2002, 'Precautions in the use of ^110MAG as a tracer of silver metabolism in ecotoxicology: Preferential bioconcentration of ¹⁰⁹CD by trout gills after ^110MAG exposure', ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, vol. 21, no. 5, pp. 1004-1008. https://doi.org/10.1897/1551-5028(2002)021<1004:PITUOA>2.0.CO;2

Precautions in the use of ^110MAG as a tracer of silver metabolism in ecotoxicology: Preferential bioconcentration of ¹⁰⁹CD by trout gills after ^110MAG exposure. / Hansen, Heinz J.M.; Grosell, Martin; Jacobsen, Ulf et al.
In: ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, Vol. 21, No. 5, 01.05.2002, p. 1004-1008.

Research output: Contribution to journal › Article › peer-review

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T2 - Preferential bioconcentration of 109CD by trout gills after 110MAG exposure

AU - Hansen, Heinz J.M.

AU - Grosell, Martin

AU - Jacobsen, Ulf

AU - Jørgensen, Jesper C.

AU - Hogstrand, Christer

AU - Wood, Christopher M.

PY - 2002/5/1

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N2 - An often overlooked problem in the use of radiotracers is the possibility of isotopic contamination. Commercially available silver 110M-Ag was used to study silver uptake and depuration in rainbow trout and European eel. Quality control by means of comparative γ and β counting brought our attention to a contamination of the 110MAg stock with 109Cd, which could be seen only because the 109Cd was markedly bioconcentrated by trout gills. The contamination could not be detected in eel gills or in other tissues of both species. The difference between trout and eel gill structure and function is the probable explanation for the marked difference in 109Cd accumulation. This contamination was identified as 109Cd by γ spectroscopy and its origin by transmutation of natural silver as a result of neutron activation is described. Failure to recognize this contamination problem would have resulted in serious misinterpretation of the data set. Guidance for avoiding this problem is given.

AB - An often overlooked problem in the use of radiotracers is the possibility of isotopic contamination. Commercially available silver 110M-Ag was used to study silver uptake and depuration in rainbow trout and European eel. Quality control by means of comparative γ and β counting brought our attention to a contamination of the 110MAg stock with 109Cd, which could be seen only because the 109Cd was markedly bioconcentrated by trout gills. The contamination could not be detected in eel gills or in other tissues of both species. The difference between trout and eel gill structure and function is the probable explanation for the marked difference in 109Cd accumulation. This contamination was identified as 109Cd by γ spectroscopy and its origin by transmutation of natural silver as a result of neutron activation is described. Failure to recognize this contamination problem would have resulted in serious misinterpretation of the data set. Guidance for avoiding this problem is given.

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