Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts

TY - JOUR

T1 - Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts

AU - Sharpe, Claire C

AU - Dockrell, Mark E C

PY - 2012

Y1 - 2012

N2 - Renal physiology and pathology are complex systems that are best studied in whole living organisms. This, however, is often restricted by our desire to limit the number of animal experiments undertaken and to replace them with relevant in vitro models that can be used as surrogates for the system under test. Primary culture cells are derived directly from the relevant tissue and therefore correlate more closely with the system under examination. Although the tissue of origin is not always readily available for culture and cells may quickly change their phenotype after only a few passages, they can be used in many circumstances to validate results obtained from closely related cell lines and to confirm vital protein expression patterns. This chapter outlines methods by which proximal tubular epithelial cells and renal interstitial fibroblasts can be isolated and characterized from human renal nephrectomy tissue.

AB - Renal physiology and pathology are complex systems that are best studied in whole living organisms. This, however, is often restricted by our desire to limit the number of animal experiments undertaken and to replace them with relevant in vitro models that can be used as surrogates for the system under test. Primary culture cells are derived directly from the relevant tissue and therefore correlate more closely with the system under examination. Although the tissue of origin is not always readily available for culture and cells may quickly change their phenotype after only a few passages, they can be used in many circumstances to validate results obtained from closely related cell lines and to confirm vital protein expression patterns. This chapter outlines methods by which proximal tubular epithelial cells and renal interstitial fibroblasts can be isolated and characterized from human renal nephrectomy tissue.

U2 - 10.1007/978-1-61779-367-7_12

DO - 10.1007/978-1-61779-367-7_12

M3 - Article

C2 - 22057452

SN - 1064-3745

VL - 806

SP - 175

EP - 185

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -