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Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia

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Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia. / Schnell, Sabine; Stott, Lucy Claire; Hogstrand, Christer; Wood, Chris; Kelly, Scott; Part, Peter; Owen, Stewart; Bury, Nicolas Richard.

In: Nature Protocols, Vol. 11, No. 3, 11.02.2016, p. 490-498.

Research output: Contribution to journalArticle

Harvard

Schnell, S, Stott, LC, Hogstrand, C, Wood, C, Kelly, S, Part, P, Owen, S & Bury, NR 2016, 'Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia', Nature Protocols, vol. 11, no. 3, pp. 490-498. https://doi.org/10.1038/nprot.2016.029

APA

Schnell, S., Stott, L. C., Hogstrand, C., Wood, C., Kelly, S., Part, P., ... Bury, N. R. (2016). Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia. Nature Protocols, 11(3), 490-498. https://doi.org/10.1038/nprot.2016.029

Vancouver

Schnell S, Stott LC, Hogstrand C, Wood C, Kelly S, Part P et al. Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia. Nature Protocols. 2016 Feb 11;11(3):490-498. https://doi.org/10.1038/nprot.2016.029

Author

Schnell, Sabine ; Stott, Lucy Claire ; Hogstrand, Christer ; Wood, Chris ; Kelly, Scott ; Part, Peter ; Owen, Stewart ; Bury, Nicolas Richard. / Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia. In: Nature Protocols. 2016 ; Vol. 11, No. 3. pp. 490-498.

Bibtex Download

@article{ff777cf71101424990a551a1dc23d22e,
title = "Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia",
abstract = "This protocol describes how to reconstruct and culture the freshwater rainbow trout gill epithelium on flat permeable membrane supports within cell culture inserts. The protocol describes gill cell isolation, cultured gill epithelium formation, maintenance, monitoring and preparation for use in experimental procedures. To produce a heterogeneous gill epithelium, as seen in vivo, seeding of isolated gill cells twice over a two day period is required. As a consequence this is termed the double seeded insert (DSI) technique. Approximately 5-12 days after cell isolation and seeding, preparations develop electrically tight gill epithelia that can withstand fresh water on the apical cell surface. The system can be used to study freshwater gill physiology, as well as a humane alternative for toxicity testing, bioaccumulation studies and environmental water quality monitoring.",
author = "Sabine Schnell and Stott, {Lucy Claire} and Christer Hogstrand and Chris Wood and Scott Kelly and Peter Part and Stewart Owen and Bury, {Nicolas Richard}",
year = "2016",
month = "2",
day = "11",
doi = "10.1038/nprot.2016.029",
language = "English",
volume = "11",
pages = "490--498",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Springer Nature",
number = "3",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia

AU - Schnell, Sabine

AU - Stott, Lucy Claire

AU - Hogstrand, Christer

AU - Wood, Chris

AU - Kelly, Scott

AU - Part, Peter

AU - Owen, Stewart

AU - Bury, Nicolas Richard

PY - 2016/2/11

Y1 - 2016/2/11

N2 - This protocol describes how to reconstruct and culture the freshwater rainbow trout gill epithelium on flat permeable membrane supports within cell culture inserts. The protocol describes gill cell isolation, cultured gill epithelium formation, maintenance, monitoring and preparation for use in experimental procedures. To produce a heterogeneous gill epithelium, as seen in vivo, seeding of isolated gill cells twice over a two day period is required. As a consequence this is termed the double seeded insert (DSI) technique. Approximately 5-12 days after cell isolation and seeding, preparations develop electrically tight gill epithelia that can withstand fresh water on the apical cell surface. The system can be used to study freshwater gill physiology, as well as a humane alternative for toxicity testing, bioaccumulation studies and environmental water quality monitoring.

AB - This protocol describes how to reconstruct and culture the freshwater rainbow trout gill epithelium on flat permeable membrane supports within cell culture inserts. The protocol describes gill cell isolation, cultured gill epithelium formation, maintenance, monitoring and preparation for use in experimental procedures. To produce a heterogeneous gill epithelium, as seen in vivo, seeding of isolated gill cells twice over a two day period is required. As a consequence this is termed the double seeded insert (DSI) technique. Approximately 5-12 days after cell isolation and seeding, preparations develop electrically tight gill epithelia that can withstand fresh water on the apical cell surface. The system can be used to study freshwater gill physiology, as well as a humane alternative for toxicity testing, bioaccumulation studies and environmental water quality monitoring.

U2 - 10.1038/nprot.2016.029

DO - 10.1038/nprot.2016.029

M3 - Article

VL - 11

SP - 490

EP - 498

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 3

ER -

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