Production of a soluble gamma delta T-cell receptor to identify ligands for the murine intestinal intraepithelial gamma delta T cell population

TY - JOUR

T1 - Production of a soluble gamma delta T-cell receptor to identify ligands for the murine intestinal intraepithelial gamma delta T cell population

AU - Steele, C R

AU - Van Remoortere, K C

AU - Hayday, A C

PY - 2003

Y1 - 2003

N2 - Although the functions and antigen recognition requirements of alphabeta T cells are well characterised, the antigens recognised by gammadelta T cells and the consequences of this recognition are unclear. gammadelta T cells are enriched within epithelia, where they eradicate transformed epithelial cells and regulate inflammation. To understand how this occurs, we need to understand the cellular ligands recognised by the gammadelta cell through the gammadelta T-cell receptor (TCR). We have therefore generated a soluble TCR (sTCR) to identify ligands for the murine gammadelta intestinal intraepithelial lymphocyte (IEL) population. sTCR was produced in the baculovirus expression system and purified by affinity chromatography on an anti-TCRdelta affinity column. sTCR was recognised by a panel of conformation-specific anti-TCR-gammadelta antibodies. We will now use our sTCR to directly test the binding of putative ligands to the TCR using surface plasmon resonance, and to isolate the ligand biochemically. (C) 2002 Elsevier Science B.V. All rights reserved.

AB - Although the functions and antigen recognition requirements of alphabeta T cells are well characterised, the antigens recognised by gammadelta T cells and the consequences of this recognition are unclear. gammadelta T cells are enriched within epithelia, where they eradicate transformed epithelial cells and regulate inflammation. To understand how this occurs, we need to understand the cellular ligands recognised by the gammadelta cell through the gammadelta T-cell receptor (TCR). We have therefore generated a soluble TCR (sTCR) to identify ligands for the murine gammadelta intestinal intraepithelial lymphocyte (IEL) population. sTCR was produced in the baculovirus expression system and purified by affinity chromatography on an anti-TCRdelta affinity column. sTCR was recognised by a panel of conformation-specific anti-TCR-gammadelta antibodies. We will now use our sTCR to directly test the binding of putative ligands to the TCR using surface plasmon resonance, and to isolate the ligand biochemically. (C) 2002 Elsevier Science B.V. All rights reserved.

M3 - Conference paper

VL - 786

SP - 297

EP - 304

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences

IS - 1-2

T2 - European Workshop on From Gene to Functional Protein

Y2 - 1 January 2003

ER -