@article{0b8a1601f2de46ce990696b951938ebb,
title = "Profiling protein expression in Klebsiella pneumoniae with a carbohydrate-based covalent probe",
abstract = "We report the application of a covalent probe based on a D-glucosamine scaffold for the profiling of the bacterial pathogen Klebsiella pneumoniae. Incubation of K. pneumoniae lysates with the probe followed by electrophoretic separation and in-gel fluorescence detection allowed the generation of strain-specific signatures and the differentiation of a carbapenem-resistant strain. The labelling profile of the probe was independent of its anomeric configuration and included several low-abundance proteins not readily detectable by conventional protein staining. Initial target identification experiments by mass spectrometry suggest that target proteins include several carbohydrate-recognising proteins, which indicates that the sugar scaffold may have a role for target recognition.",
keywords = "Antimicrobial resistance, Bacterial profiling, Carbohydrate, Covalent probe, Klebsiella",
author = "Metier, {Camille C.} and Jiaming Peng and Yong Xu and Hayley Wootton and Victoire Riesi and Steven Lynham and Sirine Zadi and Carrie Turner and Wand, {Matthew E.} and {Mark Sutton}, J. and Wagner, {Gerd K.}",
note = "Funding Information: We thank King's College London for a studentship (to CCM) and a King's China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King's College London) for recording the NMR spectra of 5-?. This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1). Funding Information: We thank King{\textquoteright}s College London for a studentship (to CCM) and a King{\textquoteright}s China Award (to YX), the EPSRC National Mass Spectrometry Facility, Swansea, for the recording of mass spectra, and Dr Andrew Atkinson (King{\textquoteright}s College London) for recording the NMR spectra of 5-β . This work was supported by an EPSRC Impact Acceleration Award (EP/R511559/1). Publisher Copyright: {\textcopyright} 2020 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2021",
month = jan,
day = "15",
doi = "10.1016/j.bmc.2020.115900",
language = "English",
volume = "30",
journal = "Bioorganic and Medicinal Chemistry",
issn = "0968-0896",
publisher = "Elsevier Limited",
}
