Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2

Sophie Péron, Leo M Miyakoshi, Monika S Brill, Diana Manzano-Franco, Julia Serrano-López, Wenqiang Fan, Nicolas Marichal Negrin, Alexander Ghanem, Karl-Klaus Conzelmann, Marisa Karow, Felipe Ortega, Sergio Gascón, Benedikt Berninger

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Abstract

While adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny towards a glutamatergic fate. Retrovirus-mediated expression of Neurog2 induced the glutamatergic lineage markers TBR2 and TBR1 in cultured SEZ progenitors which differentiated into functional glutamatergic neurons. Likewise, Neurog2-transduced SEZ progenitors acquired glutamatergic neuron hallmarks in vivo. Intriguingly, they failed to migrate towards the olfactory bulb, and instead differentiated within the SEZ or the adjacent striatum, where they received connections from local neurons as indicated by rabies virus-mediated monosynaptic tracing. In contrast, lentivirus-mediated expression of Neurog2
failed to reprogram early SEZ neurons, which maintained GABAergic identity and migrated to the olfactory bulb. Our data show that NEUROG2 can program SEZ progenitors towards a glutamatergic identity, but fails to reprogram their neuronal progeny.
Original languageEnglish
JournalStem Cell Reports
Early online date22 Nov 2023
Publication statusE-pub ahead of print - 22 Nov 2023

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