Protein quantification relevant to antidoping

TY - JOUR

T1 - Protein quantification relevant to antidoping

AU - Moncrieffe, Danielle

PY - 2020/6/1

Y1 - 2020/6/1

N2 - Accurate protein quantification of IGF-I and P-III-NP in blood is needed for antidoping testing, especially with the desire to monitor these proteins in the ‘athlete biological passport’. The development of LC–MS methodologies can be complicated and often require incorporation of appropriate reference standards. In addition to validation within a laboratory, LC–MS methods should be assessed for inter-laboratory agreement as well as compared across blood matrices to extend the scope and applicability of biological analysis. Hence, it is anticipated that in the not too distant future LC–MS methodologies will become the Gold Standard approach for protein quantification in antidoping and will effectively replace immunoassays.

AB - Accurate protein quantification of IGF-I and P-III-NP in blood is needed for antidoping testing, especially with the desire to monitor these proteins in the ‘athlete biological passport’. The development of LC–MS methodologies can be complicated and often require incorporation of appropriate reference standards. In addition to validation within a laboratory, LC–MS methods should be assessed for inter-laboratory agreement as well as compared across blood matrices to extend the scope and applicability of biological analysis. Hence, it is anticipated that in the not too distant future LC–MS methodologies will become the Gold Standard approach for protein quantification in antidoping and will effectively replace immunoassays.

KW - antidoping

KW - liquid chromatography

KW - mass spectrometry

KW - protein quantification

UR - http://www.scopus.com/inward/record.url?scp=85088211370&partnerID=8YFLogxK

U2 - 10.4155/bio-2020-0042

DO - 10.4155/bio-2020-0042

M3 - Review article

C2 - 32209021

AN - SCOPUS:85088211370

SN - 1757-6180

VL - 12

SP - 711

EP - 714

JO - Bioanalysis

JF - Bioanalysis

IS - 11

ER -