Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis

TY - JOUR

T1 - Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis

AU - Lussignol, Marion

AU - Kopp, Martina

AU - Molloy, Kelly

AU - Vizcay-Barrena, Gema

AU - Fleck, Roland A.

AU - Dorner, Marcus

AU - Bell, Kierstin L.

AU - Chait, Brian T.

AU - Rice, Charles M.

AU - Catanese, Maria Teresa

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Hepatitis C virus (HCV) is a unique enveloped virus that assembles as a hybrid lipoviral particle by tightly interacting with host lipoproteins. As a result, HCV virions display a characteristic low buoyant density and a deceiving coat, with host-derived apolipoproteins masking viral epitopes. We previously described methods to produce high-titer preparations of HCV particles with tagged envelope glycoproteins that enabled ultrastructural analysis of affinity-purified virions. Here, we performed proteomics studies of HCV isolated from culture media of infected hepatoma cells to define viral and host-encoded proteins associated with mature virions. Using two different affinity purification protocols, we detected four viral and 46 human cellular proteins specifically copurifying with extracellular HCV virions. We determined the C terminus of the mature capsid protein and reproducibly detected low levels of the viral nonstructural protein, NS3. Functional characterization of virion-associated host factors by RNAi identified cellular proteins with either proviral or antiviral roles. In particular, we discovered a novel interaction between HCV capsid protein and the nucleoporin Nup98 at cytosolic lipid droplets that is important for HCV propagation. These results provide the first comprehensive view to our knowledge of the protein composition of HCV and new insights into the complex virus-host interactions underlying HCV infection.

AB - Hepatitis C virus (HCV) is a unique enveloped virus that assembles as a hybrid lipoviral particle by tightly interacting with host lipoproteins. As a result, HCV virions display a characteristic low buoyant density and a deceiving coat, with host-derived apolipoproteins masking viral epitopes. We previously described methods to produce high-titer preparations of HCV particles with tagged envelope glycoproteins that enabled ultrastructural analysis of affinity-purified virions. Here, we performed proteomics studies of HCV isolated from culture media of infected hepatoma cells to define viral and host-encoded proteins associated with mature virions. Using two different affinity purification protocols, we detected four viral and 46 human cellular proteins specifically copurifying with extracellular HCV virions. We determined the C terminus of the mature capsid protein and reproducibly detected low levels of the viral nonstructural protein, NS3. Functional characterization of virion-associated host factors by RNAi identified cellular proteins with either proviral or antiviral roles. In particular, we discovered a novel interaction between HCV capsid protein and the nucleoporin Nup98 at cytosolic lipid droplets that is important for HCV propagation. These results provide the first comprehensive view to our knowledge of the protein composition of HCV and new insights into the complex virus-host interactions underlying HCV infection.

KW - Enveloped virus

KW - Flaviviridae

KW - Nucleoporin

KW - Virion proteomics

KW - Virus-host interactions

UR - http://www.scopus.com/inward/record.url?scp=84959284546&partnerID=8YFLogxK

U2 - 10.1073/pnas.1518934113

DO - 10.1073/pnas.1518934113

M3 - Article

AN - SCOPUS:84959284546

SN - 0027-8424

VL - 113

SP - 2484

EP - 2489

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 9

ER -