Protocol for intervention-free quantification of protein turnover rate by steady-state modeling

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Protein turnover rate is difficult to obtain experimentally. This protocol shows how to mathematically model turnover rates in an intervention-free manner given the ability to quantify mRNA and protein expression from initiation to homeostasis. This approach can be used to calculate production and degradation rates and to infer protein half-life. This model was successfully employed to quantify turnover during Drosophila embryogenesis, and we hypothesize that it will be applicable to diverse in vivo or in vitro systems.
Original languageEnglish
Article number100377
JournalSTAR Protocols
Volume2
Issue number1
DOIs
Publication statusPublished - 19 Mar 2021

Fingerprint

Dive into the research topics of 'Protocol for intervention-free quantification of protein turnover rate by steady-state modeling'. Together they form a unique fingerprint.

Cite this