King's College London

Research portal

Protocol for Patch-Seq of Small Interneurons

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Article number100146
JournalSTAR Protocols
Volume1
Issue number3
DOIs
Published18 Dec 2020

Bibliographical note

Funding Information: This work was supported by a Leverhulme Trust Research Grant (RPG-2016-095) and a Consolidator Grant from the European Research Council (725729; FUNCOPLAN). We thank Andres Crespo for technical support; Iain Macaulay and James Lipscombe at the Earlham Institute for support with the G&T protocol; and Jonathan Mill, Aaron Jeffreys, and Karen Moore at the University of Exeter for support with sequencing. Conceptualization, M.S.G.; Methodology, M.L. and L.B.; Investigation, M.L. and L.B.; Resources, M.S.G.; Writing ? Original Draft, M.L. L.B. and M.S.G.; Writing ? Review & Editing, M.L. L.B. and M.S.G.; Visualization, M.L. and L.B.; Supervision, M.S.G.; Project Administration, M.L. L.B. and M.S.G.; Funding Acquisition, M.S.G. The authors declare no competing interests. Publisher Copyright: © 2020 The Author(s) Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

King's Authors

Abstract

Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type.

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454