Ptychographic imaging of NaD1 induced yeast cell death

TY - JOUR

T1 - Ptychographic imaging of NaD1 induced yeast cell death

AU - Anthony, Nicholas

AU - Darmanin, Connie

AU - Bleackley, Mark R.

AU - Parisi, Kathy

AU - Cadenazzi, Guido

AU - Holmes, Susannah

AU - Anderson, Marilyn A.

AU - Nugent, Keith A.

AU - Abbey, Brian

N1 - Funding Information: Australian Research Council (CE140100011, DP150104386). Funding Information: 1Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia 2Nanophysics & NIC@IIT, Istituto Italiano Di Tecnologia, Via Enrico Melen 83, 16152 Genoa, Italy 3Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia 4Australian National University, ACT 0200, Australia Publisher Copyright: © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

PY - 2019

Y1 - 2019

N2 - Characterising and understanding the mechanisms involved in cell death are especially important to combating threats to human health, particularly for the study of antimicrobial peptides and their effectiveness against pathogenic fungi. However, imaging these processes often relies on the use of synthetic molecules which bind to specific cellular targets to produce contrast. Here we study yeast cell death, induced by the anti-fungal peptide, NaD1. By treating yeast as a model organism we aim to understand anti-fungal cell death processes without relying on sample modification. Using a quantitative phase imaging technique, ptychography, we were able to produce label free images of yeast cells during death and use them to investigate the mode of action of NaD1. Using this technique we were able to identify a significant phase shift which provided a clear signature of yeast cell death. Additionally, ptychography identifies cell death much earlier than a comparative fluorescence study, providing new insights into the cellular changes that occur during cell death. The results indicate ptychography has great potential as a means of providing additional information about cellular processes which otherwise may be masked by indirect labelling approaches.

AB - Characterising and understanding the mechanisms involved in cell death are especially important to combating threats to human health, particularly for the study of antimicrobial peptides and their effectiveness against pathogenic fungi. However, imaging these processes often relies on the use of synthetic molecules which bind to specific cellular targets to produce contrast. Here we study yeast cell death, induced by the anti-fungal peptide, NaD1. By treating yeast as a model organism we aim to understand anti-fungal cell death processes without relying on sample modification. Using a quantitative phase imaging technique, ptychography, we were able to produce label free images of yeast cells during death and use them to investigate the mode of action of NaD1. Using this technique we were able to identify a significant phase shift which provided a clear signature of yeast cell death. Additionally, ptychography identifies cell death much earlier than a comparative fluorescence study, providing new insights into the cellular changes that occur during cell death. The results indicate ptychography has great potential as a means of providing additional information about cellular processes which otherwise may be masked by indirect labelling approaches.

UR - http://www.scopus.com/inward/record.url?scp=85076634445&partnerID=8YFLogxK

U2 - 10.1364/BOE.10.004964

DO - 10.1364/BOE.10.004964

M3 - Article

AN - SCOPUS:85076634445

SN - 2156-7085

VL - 10

SP - 4964

EP - 4974

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 10

ER -