Purification and Immunophenotypic Characterization of Human CD24hiCD38hi and CD24hiCD27+ Regulatory B Cells in Transplantation

Rowa Y. Alhabbab; Giovanna Lombardi

doi:10.1007/978-1-0716-1237-8_24

Purification and Immunophenotypic Characterization of Human CD24^hiCD38^hi and CD24^hiCD27⁺ Regulatory B Cells in Transplantation

Rowa Y. Alhabbab, Giovanna Lombardi

Peter Gorer Department of Immunobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Transplantation is still the treatment of choice for organ failure; however, allograft induces inflammatory immune responses that require immunosuppressive treatment. The role of regulatory B cells (Bregs) in downregulating inflammation has been reported to be significant in several diseases including transplant rejection. Many reports have analyzed different B-cell subpopulations, including Bregs, in tolerant, stable, and rejecting transplant recipients as well as the influence of immunosuppressant on the frequencies and functions of the different B-cell subsets. In this chapter, the key techniques required to investigate human Breg frequencies and functions in transplant patients are discussed.

Original language	English
Pages (from-to)	451-467
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2270
DOIs	https://doi.org/10.1007/978-1-0716-1237-8_24
Publication status	Published - 2021

Keywords

Breg frequency
Breg function
CD24hiCD27+ Bregs
CD24hiCD38hi Bregs
Human Bregs
Transplantation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-1237-8_24

Cite this

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title = "Purification and Immunophenotypic Characterization of Human CD24hiCD38hi and CD24hiCD27+ Regulatory B Cells in Transplantation",

abstract = "Transplantation is still the treatment of choice for organ failure; however, allograft induces inflammatory immune responses that require immunosuppressive treatment. The role of regulatory B cells (Bregs) in downregulating inflammation has been reported to be significant in several diseases including transplant rejection. Many reports have analyzed different B-cell subpopulations, including Bregs, in tolerant, stable, and rejecting transplant recipients as well as the influence of immunosuppressant on the frequencies and functions of the different B-cell subsets. In this chapter, the key techniques required to investigate human Breg frequencies and functions in transplant patients are discussed.",

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KW - Breg frequency

KW - Breg function

KW - CD24hiCD27+ Bregs

KW - CD24hiCD38hi Bregs

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