@article{4477a5c59e0249c0aeb2d6b42537f476,
title = "Quantitative analysis of the cardiac phosphoproteome in response to acute β‐adrenergic receptor stimulation in vivo",
abstract = "β‐adrenergic receptor (β‐AR) stimulation represents a major mechanism of modulating cardiac output. In spite of its fundamental importance, its molecular basis on the level of cell signalling has not been characterised in detail yet. We employed mass spectrometry‐based proteome and phosphoproteome analysis using SuperSILAC (spike‐in stable isotope labelling by amino acids in cell culture) standardization to generate a comprehensive map of acute phosphoproteome changes in mice upon administration of isoprenaline (ISO), a synthetic β‐AR agonist that targets both β1‐AR and β2‐AR subtypes. Our data describe 8597 quantitated phosphopeptides corresponding to 10,164 known and novel phospho‐events from 2975 proteins. In total, 197 of these phospho‐events showed significantly altered phosphorylation, indicating an intricate signalling network activated in response to β‐AR stimulation. In addition, we unexpectedly detected significant cardiac expression and ISO‐induced fragmentation of junctophilin‐1, a junctophilin isoform hitherto only thought to be expressed in skeletal muscle. Data are available via ProteomeXchange with identifier PXD025569.",
keywords = "Cell signalling, Mass spectrometry, Phosphorylation, SILAC, β‐adrenergic receptor",
author = "Alican G{\"u}ran and Yanlong Ji and Pan Fang and Pan, {Kuan Ting} and Henning Urlaub and Metin Avkiran and Christof Lenz",
note = "Funding Information: A.G. was supported by a PhD studentship from the King?s College London British Heart Foundation (BHF) Research Excellence Award (RE/13/2/30182) and participated in the International Research Training Group 1816 program with the University of G?ttingen that is cofunded by the Deutsche Forschungsgemeinschaft (DFG). Y.J. and K.T.P. were supported in part by the LOEWE Center Frankfurt Cancer Institute (FCI) funded by the Hessen State Ministry for Higher Education, Research and the Arts [III L 5?519/03/03.001?(0015)]. Y.L.: P.F., H.U. and C.L. would like to acknowledge Monika Raabe, Sabine K?-nig and Uwe Plessmann at the Max Planck Institute for Biophysical Chemistry, G?ttingen, Germany for expert technical assistance. Funding Information: Funding: A.G. was supported by a PhD studentship from the King{\textquoteright}s College London British Heart Foundation (BHF) Research Excellence Award (RE/13/2/30182) and participated in the International Research Training Group 1816 program with the University of G{\"o}ttingen that is cofunded by the Deutsche Forschungsgemeinschaft (DFG). Y.J. and K.T.P. were supported in part by the LOEWE Center Frankfurt Cancer Institute (FCI) funded by the Hessen State Ministry for Higher Education, Research and the Arts [III L 5‐519/03/03.001‐(0015)]. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = nov,
day = "1",
doi = "10.3390/ijms222212584",
language = "English",
volume = "22",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "22",
}