Quantitative live cell FLIM imaging in three dimensions

Alix Le Marois, Klaus Suhling*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

16 Citations (Scopus)

Abstract

In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages31-48
Number of pages18
Volume1035
DOIs
Publication statusPublished - 28 Oct 2017

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1035
ISSN (Print)00652598
ISSN (Electronic)22148019

Keywords

  • FLIM data analysis
  • FLIM techniques
  • Multi-channel FLIM
  • Multi-modal FLIM
  • Optical sectioning
  • Time-correlated single photon counting (TCSPC)

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