Quantitative live cell FLIM imaging in three dimensions

Alix Le Marois, Klaus Suhling*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

16 Citations (Scopus)


In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Number of pages18
Publication statusPublished - 28 Oct 2017

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)00652598
ISSN (Electronic)22148019


  • FLIM data analysis
  • FLIM techniques
  • Multi-channel FLIM
  • Multi-modal FLIM
  • Optical sectioning
  • Time-correlated single photon counting (TCSPC)


Dive into the research topics of 'Quantitative live cell FLIM imaging in three dimensions'. Together they form a unique fingerprint.

Cite this