Rapid actin transport during cell protrusion

TY - JOUR

T1 - Rapid actin transport during cell protrusion

AU - Zicha, D

AU - Dobbie, I M

AU - Holt, M R

AU - Monypenny, J

AU - Soong, D Y H

AU - Gray, C

AU - Dunn, G A

PY - 2003/4/4

Y1 - 2003/4/4

N2 - Transformed rat fibroblasts expressing two variants of green fluorescent protein, each fused to beta-actin, were used to study actin dynamics during cell protrusion. The recently developed FLAP (fluorescence localization after photobleaching) method permits the tracking of one fluorophore after localized photobleaching by using the other as a colocalized reference. Here, by visualizing the ratio of bleached to total molecules, we found that actin was delivered to protruding zones of the leading edge of the cell at speeds that exceeded 5 micrometers per second. Monte Carlo modeling confirmed that this flow cannot be explained by diffusion and may involve active transport.

AB - Transformed rat fibroblasts expressing two variants of green fluorescent protein, each fused to beta-actin, were used to study actin dynamics during cell protrusion. The recently developed FLAP (fluorescence localization after photobleaching) method permits the tracking of one fluorophore after localized photobleaching by using the other as a colocalized reference. Here, by visualizing the ratio of bleached to total molecules, we found that actin was delivered to protruding zones of the leading edge of the cell at speeds that exceeded 5 micrometers per second. Monte Carlo modeling confirmed that this flow cannot be explained by diffusion and may involve active transport.

UR - https://www.scopus.com/pages/publications/0242500288

U2 - 10.1126/science.1082026

DO - 10.1126/science.1082026

M3 - Article

SN - 1095-9203

VL - 300

SP - 142

EP - 145

JO - Science

JF - Science

IS - 5616

ER -