Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals

Antoni J Borysik; Loic Briand; Andrew J Taylor; David J Scott

doi:10.1016/j.jmb.2010.09.019

Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals

Antoni J Borysik, Loic Briand, Andrew J Taylor, David J Scott

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We have measured the effect of rat odorant-binding protein 1 on the rates of ligand uptake and liquid-to-air transfer rates with a set of defined odorous compounds. Comparison of observed rate constants (k(obs)) with data simulated over a wide range of different kinetic and thermodynamic regimes shows that the data do not agree with the previously held view of a slow off-rate regime (k(off) <0.0004 s(-1)). We propose that a rapid k(off) would be a necessary requirement for such a system, since slow odorant-release rates would result in significant decorrelation between the olfactory world and odour perception.

Original language	English
Pages (from-to)	372-80
Number of pages	9
Journal	Journal of Molecular Biology
Volume	404
Issue number	3
DOIs	https://doi.org/10.1016/j.jmb.2010.09.019
Publication status	Published - 3 Dec 2010

Keywords

Acetates
Animals
Calorimetry
In Vitro Techniques
Kinetics
Ligands
Mass Spectrometry
Odors
Rats
Receptors, Odorant
Recombinant Proteins
Signal Transduction
Smell
Thermodynamics

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10.1016/j.jmb.2010.09.019

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title = "Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals",

abstract = "We have measured the effect of rat odorant-binding protein 1 on the rates of ligand uptake and liquid-to-air transfer rates with a set of defined odorous compounds. Comparison of observed rate constants (k(obs)) with data simulated over a wide range of different kinetic and thermodynamic regimes shows that the data do not agree with the previously held view of a slow off-rate regime (k(off) <0.0004 s(-1)). We propose that a rapid k(off) would be a necessary requirement for such a system, since slow odorant-release rates would result in significant decorrelation between the olfactory world and odour perception.",

keywords = "Acetates, Animals, Calorimetry, In Vitro Techniques, Kinetics, Ligands, Mass Spectrometry, Odors, Rats, Receptors, Odorant, Recombinant Proteins, Signal Transduction, Smell, Thermodynamics",

author = "Borysik, {Antoni J} and Loic Briand and Taylor, {Andrew J} and Scott, {David J}",

year = "2010",

month = dec,

day = "3",

doi = "10.1016/j.jmb.2010.09.019",

language = "English",

volume = "404",

pages = "372--80",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals

AU - Borysik, Antoni J

AU - Briand, Loic

AU - Taylor, Andrew J

AU - Scott, David J

PY - 2010/12/3

Y1 - 2010/12/3

N2 - We have measured the effect of rat odorant-binding protein 1 on the rates of ligand uptake and liquid-to-air transfer rates with a set of defined odorous compounds. Comparison of observed rate constants (k(obs)) with data simulated over a wide range of different kinetic and thermodynamic regimes shows that the data do not agree with the previously held view of a slow off-rate regime (k(off) <0.0004 s(-1)). We propose that a rapid k(off) would be a necessary requirement for such a system, since slow odorant-release rates would result in significant decorrelation between the olfactory world and odour perception.

AB - We have measured the effect of rat odorant-binding protein 1 on the rates of ligand uptake and liquid-to-air transfer rates with a set of defined odorous compounds. Comparison of observed rate constants (k(obs)) with data simulated over a wide range of different kinetic and thermodynamic regimes shows that the data do not agree with the previously held view of a slow off-rate regime (k(off) <0.0004 s(-1)). We propose that a rapid k(off) would be a necessary requirement for such a system, since slow odorant-release rates would result in significant decorrelation between the olfactory world and odour perception.

KW - Acetates

KW - Animals

KW - Calorimetry

KW - In Vitro Techniques

KW - Kinetics

KW - Ligands

KW - Mass Spectrometry

KW - Odors

KW - Rats

KW - Receptors, Odorant

KW - Recombinant Proteins

KW - Signal Transduction

KW - Smell

KW - Thermodynamics

U2 - 10.1016/j.jmb.2010.09.019

DO - 10.1016/j.jmb.2010.09.019

M3 - Article

C2 - 20932975

SN - 0022-2836

VL - 404

SP - 372

EP - 380

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 3

ER -

Rapid odorant release in mammalian odour binding proteins facilitates their temporal coupling to odorant signals

Abstract

Keywords

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