Rapid quantification of quinine and its major metabolite (3S)-3-hydroxyquinine in diluted urine by UPLC-MS/MS

TY - JOUR

T1 - Rapid quantification of quinine and its major metabolite (3S)-3-hydroxyquinine in diluted urine by UPLC-MS/MS

AU - Heaton, James

AU - Rahmioglu, Nilufer

AU - Rasekh Ahmadi, Kourosh

AU - Legido-Quigley, Cristina

AU - Smith, Norman W.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - A rapid UPLC-MS/MS quantitative assay for the quantification of quinine and (35)-3-hydroxyquinine requiring minimal sample pre-treatment - dilute-and-shoot type approach - has been developed. The assay was run at 0.6 mL/min using gradient elution with (pH 10; 10 mM) ammonium bicarbonate and methanol with a total cycle time of 2.5 min on a 50 mm x 2.1 mm ID, 1.7 mu m Acquity BEH column. Peak shapes were highly symmetrical allowing for accurate peak integration. Calibration curves for both analytes were constructed from 1.00 to 20.00 ng/mL, yielding R-2 values >0.995. lntra- and inter-batch assay precision and accuracy were evaluated using 6 injections of QC solutions on 3 separate days (n=18) and were found to be within +/- 10% and 90-110% respectively. The method was shown to be suitable for quantitatively determining the ratio of quinine to (3S)-3-hydroxyquinine for a cohort of samples from an epidemiological study. (C) 2011 Elsevier B.V. All rights reserved.

AB - A rapid UPLC-MS/MS quantitative assay for the quantification of quinine and (35)-3-hydroxyquinine requiring minimal sample pre-treatment - dilute-and-shoot type approach - has been developed. The assay was run at 0.6 mL/min using gradient elution with (pH 10; 10 mM) ammonium bicarbonate and methanol with a total cycle time of 2.5 min on a 50 mm x 2.1 mm ID, 1.7 mu m Acquity BEH column. Peak shapes were highly symmetrical allowing for accurate peak integration. Calibration curves for both analytes were constructed from 1.00 to 20.00 ng/mL, yielding R-2 values >0.995. lntra- and inter-batch assay precision and accuracy were evaluated using 6 injections of QC solutions on 3 separate days (n=18) and were found to be within +/- 10% and 90-110% respectively. The method was shown to be suitable for quantitatively determining the ratio of quinine to (3S)-3-hydroxyquinine for a cohort of samples from an epidemiological study. (C) 2011 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.jpba.2011.02.004

DO - 10.1016/j.jpba.2011.02.004

M3 - Article

VL - 55

SP - 494

EP - 499

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 3

ER -