Recombinant mussel protein Pvfp-5β: a potential tissue bioadhesive

Radha Santonocito, Francesca Venturella, Fabrizio Dal Piaz, Maria Agnese Morando, Alessia Provenzano, Estella Rao, Maria Assunta Costa, Donatella Bulone, Pier Luigi San Biagio, Daniela Giacomazza, Alessandro Sicorello, Caterina Alfano, Rosa Passantino, Annalisa Pastore

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26 Citations (Scopus)
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Abstract

During their lifecycle, many marine organisms rely on natural adhesives to attach to wet surfaces for movement and self-defence in aqueous tidal environments. Adhesive proteins from mussels are biocompatible and elicit only minimal immune responses in humans. Therefore these proteins have received increased attention for their potential applications in medicine, biomaterials and biotechnology. The Asian green mussel Perna viridis secretes several byssal plaque proteins, molecules that help anchor the mussel to surfaces. Among these proteins, protein-5β (Pvfp-5β) initiates interactions with the substrate, displacing interfacial water molecules before binding to the surface. Here, we established the first recombinant expression in Escherichia coli of Pvfp-5β. We characterized recombinant Pvfp-5β, finding that despite displaying a CD spectrum consistent with features of a random coil, the protein is correctly folded as indicated by MS and NMR analyses. Pvfp-5β folds as a β-sheet–rich protein as expected for an epidermal growth factor (EGF)-like module. We examined the effects of Pvfp-5β on cell viability and adhesion capacity in NIH-3T3 and HeLa cell lines, revealing that Pvfp-5β has no cytotoxic effects at the protein concentrations used and provides good cell-adhesion strength on both glass and plastic plates. Our findings suggest that the adhesive properties of recombinant Pvfp-5β make it an efficient surface coating material, potentially suitable for biomedical applications including regeneration of damaged tissues.
Original languageEnglish
Article numberjbc.RA119.009531
Pages (from-to)12826-12835
Number of pages10
JournalJournal of Biological Chemistry
Volume294
Issue number34
Early online date10 Jul 2019
DOIs
Publication statusE-pub ahead of print - 10 Jul 2019

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