Regulation of alternative polyadenylation by genomic imprinting

A J Wood, R Schulz, K Woodfine, K Koltowska, C V Beechey, J Peters, D Bourc'his, R J Oakey

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)

Abstract

Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in co-transcriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate truncated H13 transcripts that undergo internal polyadenylation. On methylated alleles, the internal promoter is inactive and elongation proceeds to downstream polyadenylation sites. This demonstrates that epigenetic modifications can influence utilization of alternative polyadenylation sites
Original languageEnglish
Pages (from-to)1141 - 1146
Number of pages6
JournalGenes and Development
Volume22
Issue number9
DOIs
Publication statusPublished - 1 May 2008

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