Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron

D M Johnson, S Yamaji, J Tennant, S K Srai, P A Sharp

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4 h exhibited a significant decrease in plasma membrane DMTI protein, though total cellular DMTI levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMTI in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMTI transporter protein towards a late endosomal/lysosomal compartment. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved
Original languageEnglish
Pages (from-to)1923 - 1929
Number of pages7
JournalFEBS Letters
Volume579
Issue number9
DOIs
Publication statusPublished - 28 Mar 2005

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