Regulation of Glucose Transporter Expression in Human Intestinal Caco-2 Cells following Exposure to an Anthocyanin-Rich Berry Extract

Fawaz Alzaid, Hoi-Man Cheung, Victor R. Preedy*, Paul A. Sharp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)

Abstract

Polyphenols contained within plant tissues are consumed in significant amounts in the human diet and are known to influence a number of biological processes. This study investigated the effects of an anthocyanin-rich berry-extract on glucose uptake by human intestinal Caco-2 cells. Acute exposure (15 min) to berry extract (0.125%, w/v) significantly decreased both sodium-dependent (Total uptake) and sodium-independent (facilitated uptake) H-3-D-glucose uptake. In longer-term studies, SGLT1 mRNA and GLUT2 mRNA expression were reduced significantly. Polyphenols are known to interact directly with glucose transporters to regulate the rate of glucose absorption. Our in vitro data support this mechanism and also suggest that berry flavonoids may modulate post-prandial glycaemia by decreasing glucose transporter expression. Further studies are warranted to investigate the longer term effects of berry flavonoids on the management of glycaemia in human volunteers.

Original languageEnglish
Article numbere78932
Number of pages6
JournalPL o S One
Volume8
Issue number11
DOIs
Publication statusPublished - 13 Nov 2013

Keywords

  • RAT SMALL-INTESTINE
  • SUGAR ABSORPTION
  • DIFFERENTIAL EXPRESSION
  • DIFFUSIVE COMPONENT
  • DIETARY POLYPHENOLS
  • EPITHELIAL-CELLS
  • APICAL GLUT2
  • SGLT1
  • MICE
  • ENTEROCYTES

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