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Regulatory T cell-derived extracellular vesicles modify dendritic cell function

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Regulatory T cell-derived extracellular vesicles modify dendritic cell function. / Fanelli, Giorgia; Lombardi, Giovanna; Tung, Sim Lai; Al-Jamal, Khuloud; Smyth, L. A.

In: Frontiers in Cell and Developmental Biology, Vol. 8, No. 1, 6065, 20.05.2020.

Research output: Contribution to journalArticle

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Fanelli, G, Lombardi, G, Tung, SL, Al-Jamal, K & Smyth, LA 2020, 'Regulatory T cell-derived extracellular vesicles modify dendritic cell function', Frontiers in Cell and Developmental Biology, vol. 8, no. 1, 6065. https://doi.org/10.1038/s41598-018-24531-8

APA

Fanelli, G., Lombardi, G., Tung, S. L., Al-Jamal, K., & Smyth, L. A. (2020). Regulatory T cell-derived extracellular vesicles modify dendritic cell function. Frontiers in Cell and Developmental Biology, 8(1), [6065]. https://doi.org/10.1038/s41598-018-24531-8

Vancouver

Fanelli G, Lombardi G, Tung SL, Al-Jamal K, Smyth LA. Regulatory T cell-derived extracellular vesicles modify dendritic cell function. Frontiers in Cell and Developmental Biology. 2020 May 20;8(1). 6065. https://doi.org/10.1038/s41598-018-24531-8

Author

Fanelli, Giorgia ; Lombardi, Giovanna ; Tung, Sim Lai ; Al-Jamal, Khuloud ; Smyth, L. A. / Regulatory T cell-derived extracellular vesicles modify dendritic cell function. In: Frontiers in Cell and Developmental Biology. 2020 ; Vol. 8, No. 1.

Bibtex Download

@article{098a0ff79f2b4b229b721e304ff16bf0,
title = "Regulatory T cell-derived extracellular vesicles modify dendritic cell function",
abstract = "Regulatory T cells (Treg) are a subpopulation of T cells that maintain tolerance to self and limit other immune responses. They achieve this through different mechanisms including the release of extracellular vesicles (EVs) such as exosomes as shown by us, and others. One of the ways that Treg derived EVs inhibit target cells such as effector T cells is via the transfer of miRNA. Another key target for the immunoregulatory function of Tregs is the dendritic cells (DCs). In this study we demonstrate directly, and for the first time, that miRNAs are transferred from Tregs to DCs via Treg derived EVs. In particular two miRNAs, namely miR-150-5p and miR-142-3p, were increased in DCs following their interaction with Tregs and Treg derived exosomes. One of the consequences for DCs following the acquisition of miRNAs contained in Treg derived EVs was the induction of a tolerogenic phenotype in these cells, with increased IL-10 and decreased IL-6 production being observed following LPS stimulation. Altogether our findings provide data to support the idea that intercellular transfer of miRNAs via EVs may be a novel mechanism by which Tregs regulate DC function and could represent a mechanism to inhibit immune reactions in tissues.",
author = "Giorgia Fanelli and Giovanna Lombardi and Tung, {Sim Lai} and Khuloud Al-Jamal and Smyth, {L. A.}",
year = "2020",
month = "5",
day = "20",
doi = "10.1038/s41598-018-24531-8",
language = "English",
volume = "8",
journal = "Frontiers in Cell and Developmental Biology",
issn = "2296-634X",
publisher = "Frontiers Media S.A.",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Regulatory T cell-derived extracellular vesicles modify dendritic cell function

AU - Fanelli, Giorgia

AU - Lombardi, Giovanna

AU - Tung, Sim Lai

AU - Al-Jamal, Khuloud

AU - Smyth, L. A.

PY - 2020/5/20

Y1 - 2020/5/20

N2 - Regulatory T cells (Treg) are a subpopulation of T cells that maintain tolerance to self and limit other immune responses. They achieve this through different mechanisms including the release of extracellular vesicles (EVs) such as exosomes as shown by us, and others. One of the ways that Treg derived EVs inhibit target cells such as effector T cells is via the transfer of miRNA. Another key target for the immunoregulatory function of Tregs is the dendritic cells (DCs). In this study we demonstrate directly, and for the first time, that miRNAs are transferred from Tregs to DCs via Treg derived EVs. In particular two miRNAs, namely miR-150-5p and miR-142-3p, were increased in DCs following their interaction with Tregs and Treg derived exosomes. One of the consequences for DCs following the acquisition of miRNAs contained in Treg derived EVs was the induction of a tolerogenic phenotype in these cells, with increased IL-10 and decreased IL-6 production being observed following LPS stimulation. Altogether our findings provide data to support the idea that intercellular transfer of miRNAs via EVs may be a novel mechanism by which Tregs regulate DC function and could represent a mechanism to inhibit immune reactions in tissues.

AB - Regulatory T cells (Treg) are a subpopulation of T cells that maintain tolerance to self and limit other immune responses. They achieve this through different mechanisms including the release of extracellular vesicles (EVs) such as exosomes as shown by us, and others. One of the ways that Treg derived EVs inhibit target cells such as effector T cells is via the transfer of miRNA. Another key target for the immunoregulatory function of Tregs is the dendritic cells (DCs). In this study we demonstrate directly, and for the first time, that miRNAs are transferred from Tregs to DCs via Treg derived EVs. In particular two miRNAs, namely miR-150-5p and miR-142-3p, were increased in DCs following their interaction with Tregs and Treg derived exosomes. One of the consequences for DCs following the acquisition of miRNAs contained in Treg derived EVs was the induction of a tolerogenic phenotype in these cells, with increased IL-10 and decreased IL-6 production being observed following LPS stimulation. Altogether our findings provide data to support the idea that intercellular transfer of miRNAs via EVs may be a novel mechanism by which Tregs regulate DC function and could represent a mechanism to inhibit immune reactions in tissues.

UR - http://www.scopus.com/inward/record.url?scp=85045769880&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-24531-8

DO - 10.1038/s41598-018-24531-8

M3 - Article

VL - 8

JO - Frontiers in Cell and Developmental Biology

JF - Frontiers in Cell and Developmental Biology

SN - 2296-634X

IS - 1

M1 - 6065

ER -

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