Research output: Contribution to journal › Article › peer-review
Sim L. Tung, Dominic A. Boardman, Monica Sen, Marilena Letizia, Qi Peng, Nicole Cianci, Laura Dioni, Leo M. Carlin, Robert Lechler, Valentina Bollati, Giovanna Lombardi, Lesley A. Smyth
Original language | English |
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Article number | 6065 |
Journal | Scientific Reports |
Volume | 8 |
Issue number | 1 |
Early online date | 17 Apr 2018 |
DOIs | |
Accepted/In press | 3 Apr 2018 |
E-pub ahead of print | 17 Apr 2018 |
Published | Dec 2018 |
Additional links |
Regulatory T cell extracellular_SMYTH_Accepted9April2020_GOLD VoR (CC BY)
fcell_08_00317.pdf, 2.38 MB, application/pdf
Uploaded date:20 May 2020
Version:Final published version
Licence:CC BY
Regulatory T cells (Treg) are a subpopulation of T cells that maintain tolerance to self and limit other immune responses. They achieve this through different mechanisms including the release of extracellular vesicles (EVs) such as exosomes as shown by us, and others. One of the ways that Treg derived EVs inhibit target cells such as effector T cells is via the transfer of miRNA. Another key target for the immunoregulatory function of Tregs is the dendritic cells (DCs). In this study we demonstrate directly, and for the first time, that miRNAs are transferred from Tregs to DCs via Treg derived EVs. In particular two miRNAs, namely miR-150-5p and miR-142-3p, were increased in DCs following their interaction with Tregs and Treg derived exosomes. One of the consequences for DCs following the acquisition of miRNAs contained in Treg derived EVs was the induction of a tolerogenic phenotype in these cells, with increased IL-10 and decreased IL-6 production being observed following LPS stimulation. Altogether our findings provide data to support the idea that intercellular transfer of miRNAs via EVs may be a novel mechanism by which Tregs regulate DC function and could represent a mechanism to inhibit immune reactions in tissues.
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